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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
| UnitDefinition | OneWeissunitofT4DNALigaseconverts1nmoleofp32frompyrophosphateintoNorit-adsorbablematerialin20minutesat37℃in33mMTris-acetate(pH7.8),66mMpotassiumacetate,10mMmagnesiumacetate,0.5mMDTT,and1mMATP.OneWeissunitequalsapproximately200cohesive-endligationunits. |
|---|---|
| QualityControl | T4DNALigaseisfunctionallytestedincloningassaysandisfreeofdetectablecontaminatingDNAexo-andendo-nucleaseandRNaseactivities. |
| Concentration | 1Weissunit/㎕ |
| StorageBuffer | 50mMTris-HCl(pH7.5),0.1MNaCl,0.1mMEDTA,0.1%TritonX-100,and1mMDTT,50%Glycerol |
| 10XReactionbuffer | composedof400mMTris-HCl,100mMMgCl2,100mMDTT,5mMATP(pH7.8at25°C). |
| Application | Cloningofrestrictionfragments |
| Joininglinkersandadapterstoblunt-endedDNA |
