Contents | amfiEco Taq DNA Polymerase, 1u/ulamfiEco 2X Reaction Buffer |
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Application | Routine Robust PCR |
amfiEcoTaq DNA Polymeraseis a thermostable DNApolymerase that possesses a 5' -> 3' polymerase activityand a double-strand specific 5' ->3' exonuclease activity.2X amfiEco PCR Master buffer including dNTPs andMg++ minimises reagents handling steps and reducethe risk of contamination.
To perform PCR, simply add water, primers and templateto resuspend the reaction mixture, and cycle.For addedconvenience,amfiEcoTaq DNA Polymerasecontainsred and yellow loading dyes to allow loading of PCRproduct directly on a gel after thermal cycling, minimizingpipetting steps and providing easy visualization of sample.The red dye runs in a range between 500bp (2% gel) and1500bp(0.8% gel) and the yellow dye runs at less than10bp.