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Gendepot/Bovine Serum Albumin,Cell Culture Tested, CA068/CA068-050-500 g

价格
¥13700.00
货号:CA068-050-500g
浏览量:70
品牌:Gendepot
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商品描述
Purity> 99.5 %
pH 6.5 - 7.5 (10% Solution in Water)
Endotoxin< 3="">
Heavy Metals(Pb)< 10="">
Albumins are an important component of many serum-free cell culture systems such as those that utilize hybridoma or Chinese Hamster Ovary (CHO) cells. Not all albumins have the same efficacy in culture media. Major factors that control the activity of albumin include the quality and relative quantity of specific ligands associated with the molecule.
The ligands associated with albumin are determined largely by the nutritional status of the source animal and the purification process. This explains why the effectiveness of albumin(s) in a given cell culture system may vary and must be controlled. The ligand profile also helps to explain why natural albumin(s) derived from human (HSA) or bovine (FBS) sera perform
differently than recombinant albumin(s) in cell culture.
Gendepot的T4 DNA连接酶是最通用的DNA克隆连接酶。这种ATP依赖性酶将5'-磷酸与3'-羟基化的末端共价连接,这些末端是通过限制性酶消化或其他酶促过程产生的双链DNA片段的平末端或相容的内聚末端。T4 DNA连接酶对单链核酸无活性。连接反应后,可通过在65℃温育10分钟使T4 DNA连接酶失活。