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Gendepot/RPMI-1640, With L-Glutamine and HEPES, CM058/20X500ml/CM058-320

价格
¥3900.00
货号:CM058-320
浏览量:58
品牌:Gendepot
服务
全国联保
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正规发票
签订合同
商品描述
D-Glucose2000 mg/L
L-Glutamine300 mg/L
HEPES5958 mg/L
Phenol Red5 mg/L
RPMI-1640 was developed in 1966 by Moore and his co-workers at Roswell Park Memorial Institute (hence the acronym RPMI). It is based on the RMPI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. While it was originally formulated to support lymphoblastoid is suspension culture, it has been proven to support a wide variety of anchorage-dependant cells. It has a wide range of uses, including the culture of fresh human lymphocytes, fusion protocols, and in the growth of hybrid cells.
Gendepot的T4 DNA连接酶是最通用的DNA克隆连接酶。这种ATP依赖性酶将5'-磷酸与3'-羟基化的末端共价连接,这些末端是通过限制性酶消化或其他酶促过程产生的双链DNA片段的平末端或相容的内聚末端。T4 DNA连接酶对单链核酸无活性。连接反应后,可通过在65℃温育10分钟使T4 DNA连接酶失活。