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蚂蚁淘在线 / 品牌中心 / Gendepot / Gendepot/McCoys 5A Media (Modified), No L-Glutamine, CM033/500ml/CM033-050
商品描述
| D-Glucoe | 3000 mg/L |
|---|---|
| L-Glutamine | No L-Glutamine |
| Bacto-Peptone | 600 mg/L |
| Phenol Red | 10 mg/L |
In 1959, McCoy and his co-workers published the amino acid requirements for the culture of Novikoff Hepatoma Cells. These studies were originally performed using Basal Medium 5A, but the medium was subsequently modified resulting in the new medium known as McCoy"s 5A Medium. Iwakata and Grace further modified the medium for use in their work and human myeloblasts.
Gendepot的T4 DNA连接酶是最通用的DNA克隆连接酶。这种ATP依赖性酶将5'-磷酸与3'-羟基化的末端共价连接，这些末端是通过限制性酶消化或其他酶促过程产生的双链DNA片段的平末端或相容的内聚末端。T4 DNA连接酶对单链核酸无活性。连接反应后，可通过在65℃温育10分钟使T4 DNA连接酶失活。 
