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Gendepot/PureSelect Protein A/G Agarose Fast-Flow, P9303/P9303-010-10ml

价格
¥10400.00
货号:P9303-010-10ml
浏览量:46
品牌:Gendepot
服务
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商品描述
LigandRecombinant Streptococcal protein A/G lacking the albumin binding sites expressed in E. coli
Binding Capacity> 20mg sheep IgG and 30mg human IgG/ml
Resin Volume90mm (45-165mm)
GenDEPOT Protein A/G-Agarose is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. The recombinant protein A/G ligand is coupled to highly cross-linked agarose. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc. Protein A/G also binds well to IgG subclasses but does not bind IgA,IgM or serum albumin. This makes Protein A/G an excellent tool for purification and detection of monoclonal antibodies from IgG subclasses, without interference from IgA, IgM and serum albumin. Individual subclasses of monoclonals are likely to have a stronger affinity to the chimeric Protein A/G than to either ProteinA or Protein G.
Gendepot的T4 DNA连接酶是最通用的DNA克隆连接酶。这种ATP依赖性酶将5'-磷酸与3'-羟基化的末端共价连接,这些末端是通过限制性酶消化或其他酶促过程产生的双链DNA片段的平末端或相容的内聚末端。T4 DNA连接酶对单链核酸无活性。连接反应后,可通过在65℃温育10分钟使T4 DNA连接酶失活。