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Gendepot/Goat anti-Rabbit IgG(H+L)-HRP, 1mg/ml, SA002/500ul/SA002-500

价格
¥1900.00
货号:SA002-500
浏览量:109
品牌:Gendepot
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商品描述
Suggested Working ConcentrationOptimal working concentration should be determined experimentally. Prepare working dilution in TBS or other buffer such as BSA or Milk Diluent/Blocking Solution.
ELISA1:1000 - 1:5000 (1.0 ug/mL - 0.2 ug/mL)
Western Blot1:5000- 1:20000 (0.2 ug/mL - 0.05 ug/mL)
Histo/Cytochemical1:200 - 1:500 (5.0 ug/mL - 2.0 ug/mL)
ApplicationELISA, Blotting,Histo/Cytochemical Procedures
Affinity purified antibody isolated from a pool ofserum from goats immunized with purified rabbit IgGwas labeled with peroxidase by the periodate method of Nakane and Kawaoi.
Gendepot的T4 DNA连接酶是最通用的DNA克隆连接酶。这种ATP依赖性酶将5'-磷酸与3'-羟基化的末端共价连接,这些末端是通过限制性酶消化或其他酶促过程产生的双链DNA片段的平末端或相容的内聚末端。T4 DNA连接酶对单链核酸无活性。连接反应后,可通过在65℃温育10分钟使T4 DNA连接酶失活。