LeptinBindingDomainChickenRecombinantalsocalledLeptinReceptorproducedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining208aminoacidsandhavingamolecularmassof24.5kDa.ChickenLeptinReceptorconsistsofthecytokinebindingdomainofleptinreceptoraminoacids420-626ofchickenleptinreceptor.TheLeptinBindingDomainispurifiedbyproprietarychr
Synonyms
OBProtein,ObesityProtein,OBS,Obesityfactor.
EscherichiaColi.SterileFilteredWhitelyophilized(freeze-dried)powder.Theproteinwaslyophilizedfromaconcentrated(1mg/ml)solutionwith0.0045mMNaHCO3.Greaterthan99.0%asdeterminedby:(a)AnalysisbySEC-HPLC.(b)AnalysisbySDS-PAGE.Lyophilizedformstableatroomtemperaturefor3weeks,
Uponreconstitution,shouldbestoredat4oCbetween2-7days
andforfutureusebelow-20oC.omatographictechniques.
Gendepot的T4 DNA连接酶是最通用的DNA克隆连接酶。这种ATP依赖性酶将5'-磷酸与3'-羟基化的末端共价连接，这些末端是通过限制性酶消化或其他酶促过程产生的双链DNA片段的平末端或相容的内聚末端。T4 DNA连接酶对单链核酸无活性。连接反应后，可通过在65℃温育10分钟使T4 DNA连接酶失活。
