Product Format:Sterile filtered colorless solution.Host:Escherichia coli:::Product Introduction: ALKBH2 is a member of the ALKB family.ALKBH2 is expressed in the heart, colon, liver, testis, ovary, prostate and the small intestine. ALKBH2 uses iron as a cofactor and acts as a dioxygenase which catalyzes the repair of alkylated DNA and RNA containing 1-methyladenine and 3-meth-ylcytosine. ALKBH2 is functionally activated by ascorbate and requires oxygen and ?-ketoglutarate for enzymatic activity.Product Description: ALKBH2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 281 amino acids (1-261 a.a.) and having a molecular mass of 31.4kDa. ALKBH2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.Reconstitution and Storage:Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.Purity:Greater than 90.0% as determined by SDS-PAGE.Peptide Sequence:MGSSHHHHHH SSGLVPRGSH MDRFLVKGAQ GGLLRKQEEQ EPTGEEPAVL GGDKESTRKR PRREAPGNGG HSAGPSWRHI RAEGLDCSYT VLFGKAEADE IFQELEKEVE YFTGALARVQ VFGKWHSVPR KQATYGDAGL TYTFSGLTLS PKPWIPVLER IRDHVSGVTG QTFNFVLINR YKDGCDHIGE HRDDERELAP GSPIASVSFG ACRDFVFRHK DSRGKSPSRR VAVVRLPLAH GSLLMMNHPT NTHWYHSLPV RKKVLAPRVN LTFRKILLTK K.Formulation:ALKBH2 solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT and 30% glycerol.Datasheets/Manuals:Printable datasheet for OOPA00125Gene Symbol:ALKBH2Alias Symbols:Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2, Alkylated DNA repair protein alkB homolog 2, Oxy DC1, ALKBH2, ABH2, FLJ99103, MGC90512. 我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物，这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌，哺乳动物细胞和昆虫细胞中表达，并作为整体裂解物提供。
