Product Format:Sterile Filtered colorless solution.Host:HEK293 cells.:::Product Introduction: The type II transmembrane protein FASLG is a member of the tumor necrosis factor (TNF) superfamily. A fas ligand/receptor interaction has a significant part in the regulation of the immune system and the advancement of cancer. FASLG is expressed on the activated T cell surface as a nondisulfidelinked homotrimer. FASLG binding to Fas/CD95/TNFRSF6 on a nearby cell prompts apoptosis in the Fas expressing cell. FASLG is released from the cell surface by metalloproteinases as a soluble molecule that stays trimeric and is able to bind with Fas, but its capability to activate apoptosis is radically reduced. In addition, FASLG binds to DcR3 - a soluble trap receptor with no signal transduction capabilities. Flawed Fas-mediated apoptosis causes oncogenesis in addition to drug resistance in existing tumors. Constitutive expression of FASLG in a variety of tumors enables their immune evasion. Both mouse and human FASLG are active on mouse and human cells.Product Description: Recombinant Human FAS Ligand produced in HEK293 cells is a non-glycosylated, polypeptide chain containing 147 amino acids (134-281a.a). FASLG is fused to a 6 amino acid His-tag at N-terminus and purified by proprietary chromatographic techniques.Reconstitution and Storage:FASLG Human Recombinant although stable at 4C for 1 week, should be stored below -18C. Please prevent freeze thaw cycles.Formulation:The FASLG solution (0.6mg/ml) contains 1xPBS.Purification:Greater than 95.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.Purity:Greater than 95.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.Biological Activity:Fas ligand is biologically active as determined by its ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The expected ED50< 10="" ng/ml,="" corresponding="" to="" a="" specific="" activity="" of="" 1x105="">Datasheets/Manuals:Printable datasheet for OOPA00062Gene Symbol:FASLGAlias Symbols:Fas ligand (TNF superfamily, member 6), APT1LG1, FASL, TNFSF6, CD178, tumor necrosis factor (ligand) superfamily member 6, Apoptosis antigen ligand, Fas antigen ligand, APTL, CD95-L. 我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物，这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌，哺乳动物细胞和昆虫细胞中表达，并作为整体裂解物提供。
