Product Format:Liquid. Foxp3 / Transcription Factor Fix/Perm Concentrate (4x) contains formaldehyde. Use appropriate personal protective equipment in order to avoid contact with skin and eyes.Application:FCAdditional Information:Description: Aviva Foxp3 / Transcription Factor Fix/Perm Concentrate (4X) is a concentrated solution which, when diluted with Foxp3 / Transcription Factor Fix/Perm Diluent (1X) (cat. no. TNB-1022-L160), provides best results in protocols for intranuclear staining of transcription factors using fluorescently conjugated antibodies, followed by flow cytometric analysis. These reagents are formulated for optimal resolution and low background in your analysis of key nuclear proteins such as Foxp3.
Reconstitution and Storage:2-8CApplication Info:After cell surface staining is complete and cells are washed, completely resuspend the cell pellet by briefly vortexing. Add 1 mL of the 1X working solution to the cells, vortex briefly, and incubate for 30-60 minutes at room temperature or 4C. Samples should be protected from light. Following the incubation period, wash cells 2 times with 1X Flow Cytometry Perm Buffer (TNB-1213-L150) prior to incubation with conjugated antibodies specific for intracellular proteins.Datasheets/Manuals:Printable datasheet for OOTA00779 我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物,这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌,哺乳动物细胞和昆虫细胞中表达,并作为整体裂解物提供。