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No optimization required. Switching to the Aviva Pico Western Substrate from other brands does not require optimization or protocol changes.
High degree of sensitivity and enhanced chemiluminescence duration. Aviva Pico Western Substrate enables an accurate low picogram or high femtogram detection of protein on the same immunoblot after a single exposure.
Optimized for use with PVDF and nitrocellulose membranes.
Compatible with Western Blotting Markers.
Optimized for film and CCD-based imaging.

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Chemiluminescent Development:

Keep the membrane moist in the wash buffer while preparing the substrate mixture. Make sure the membrane does not dry out in the next steps.
The chemiluminescent substrate solution is sufficiently stirred to prepare the 0.1ml of solution / cm2 of the membrane.
Place the membrane on a clean, flat surface or in a clean container.
Remove the membrane from the chemiluminescent substrate solution and drain the excess solution.
Place the membrane in a plastic sheet protector or plastic wrap to prevent the film from drying out.
Imaging the membrane with a digital imager or by exposure to the X-ray film.

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Performance: The membrane was probed with a 1:10000 dilution of b-actin antibody and then serially diluted HeLa whole cell lysates were prepared using a rabbit IgG IgG HRP conjugated secondary antibody at 1:10,000, and apply in electrophoresis and protein transfer. The same blot was incubated with 5 ml of Aviva Pico Western Substrate. Blots were simultaneously exposed for 10 seconds, 30 seconds and 60 seconds using a UVP BIO SPECTRUM-AC digital imaging system.

Reconstitution and Storage:

Store at room temperature for 1 year, 4C for 2 years.

Datasheets/Manuals:

Printable datasheet for OOBJ00001

我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物，这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌，哺乳动物细胞和昆虫细胞中表达，并作为整体裂解物提供。

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