Predicted Species Reactivity:BovineApplication:ELISAKit Detection Method:Colorimetric, OD450 nmKit Principle:Aviva Systems Biology CSNK2A1 ELISA Kit (Bovine) (OKEH07551) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CSNK2A1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CSNK2A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CSNK2A1 captured in well.Kit Recovery:Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 117% (n = 20)Kit Reproducibility:Mean Intra-assay CV%: <=8.3% (n="">Mean Inter-assay CV%: <=12.4% (n="">Kit Component:| Component | Amount |
|---|
| CSNK2A1 Microplate | 96 Wells (12 x 8 Well strips) |
| CSNK2A1 Lyophilized Standard | 2 |
| 100X Biotinylated CSNK2A1 Detector Antibody | 1 x 120 uL |
| 100X Avidin-HRP Conjugate | 1 x 120 uL |
| Sample Diluent | 1 x 20 mL |
| Detector Antibody Diluent | 1 x 12 mL |
| Conjugate Diluent | 1 x 12 mL |
| 25X Wash Buffer | 1 x 30 mL |
| TMB Substrate | 1 x 10 mL |
| Stop Solution | 1 x 10 mL |
Additional Information:Function: Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response. During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Also required for p53/TP53-mediated apoptosis, phosphorylating "Ser-392" of p53/TP53 following UV irradiation. Can also negatively regulate apoptosis. Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3. Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8. Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV. Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB. Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function. Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1. Acts as an ectokinase that phosphorylates several extracellular proteins (By similarity). Phosphorylates PML at "Ser-565" and primes it for ubiquitin-mediated degradation. Plays an important role in the circadian clock function by phosphorylating ARNTL/BMAL1 at "Ser-90" which is pivotal for its interaction with CLOCK and which controls CLOCK nuclear entry. Phosphorylates CCAR2 at "Thr-454".:::Subcellular Location: Nucleus:::Unigene: Bt.64603SMR: P68399:::KEGG: bta:282419Pfam: PF00069:::KEGG: bta:282419Pfam: PF00069Reconstitution and Storage:Store as indicated in product manual.Datasheets/Manuals:Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.Specificity:Natural and recombinant Bovine Casein kinase II subunit alphaAssay Info:Assay Methodology: Quantitative Sandwich ELISAGene Symbol:CSNK2A1Alias Symbols:CK II alpha, CK2A1NCBI Gene Id:282419Protein Name:casein kinase II subunit alphaDescription of Target:Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythms. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit.Swissprot Id:P68399Protein Accession #:NP_777060.2Nucleotide Accession #:NM_174635.2Tissue Tool:Find tissues and cell lines supported by DNA array analysis to express CSNK2A1. RNA Seq:Find tissues and cell lines supported by RNA-seq analysis to express CSNK2A1. 我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物，这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌，哺乳动物细胞和昆虫细胞中表达，并作为整体裂解物提供。
