Predicted Species Reactivity:DogApplication:ELISAKit Detection Method:Colorimetric, OD450 nmKit Linearity:| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 85-95% (n = 20) | 108-120% (n = 20) | 94-104% (n = 20) | 87-97% (n = 20) |
| EDTA plasma(n=5) | 107-120% (n = 20) | 85-95% (n = 20) | 87-97% (n = 20) | 93-103% (n = 20) |
| heparin plasma(n=5) | 103-115% (n = 20) | 107-117% (n = 20) | 84-94% (n = 20) | 98-107% (n = 20) |
Kit Principle:Aviva Systems Biology PTGDS ELISA Kit (Dog) (OKEH07338) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PTGDS has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PTGDS is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PTGDS captured in well.Kit Range:0.625 - 40ng/mLKit Recovery:Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 88% (n = 20)Kit Reproducibility:Mean Intra-assay CV%: <=6.8% (n="">Mean Inter-assay CV%: <=10.2% (n="">Kit Component:| Component | Amount |
|---|
| PTGDS Microplate | 96 Wells (12 x 8 Well strips) |
| PTGDS Lyophilized Standard | 2 |
| 100X Biotinylated PTGDS Detector Antibody | 1 x 120 uL |
| 100X Avidin-HRP Conjugate | 1 x 120 uL |
| Sample Diluent | 1 x 20 mL |
| Detector Antibody Diluent | 1 x 12 mL |
| Conjugate Diluent | 1 x 12 mL |
| 25X Wash Buffer | 1 x 30 mL |
| TMB Substrate | 1 x 10 mL |
| Stop Solution | 1 x 10 mL |
Additional Information:Function: Catalyzes the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. Binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophopic molecules and as a secretory retinoid and thyroid hormone transporter. Possibly involved in development and maintenance of the blood-brain, blood-retina, blood-aqueous humor and blood-testis barrier. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system.:::Subcellular Location: Rough endoplasmic reticulum Nucleus membrane Golgi apparatus Cytoplasm perinuclear region Secreted Detected on rough endoplasmic reticulum of arachnoid and menigioma cells. Localized to the nuclear envelope, Golgi apparatus, secretory vesicles and spherical cytoplasmic structures in arachnoid trabecular cells, and to circular cytoplasmic structures in meningeal macrophages and perivascular microglial cells. In oligodendrocytes, localized to the rough endoplasmic reticulum and nuclear envelope. In retinal pigment epithelial cells, localized to distinct cytoplasmic domains including the perinuclear region. Also secreted.Reconstitution and Storage:Store as indicated in product manual.Sensitivity:0.268ng/mLDatasheets/Manuals:Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.Specificity:Natural and recombinant Dog Prostaglandin-H2 D-isomeraseAssay Info:Assay Methodology: Quantitative Sandwich ELISAGene Symbol:PTGDSOfficial Gene Full Name:prostaglandin D2 synthaseAlias Symbols:Glutathione-independent PGD synthase, Lipocalin-type prostaglandin-D synthase, Prostaglandin-D2 synthase, PGD2 synthase, PGDS, PGDS2NCBI Gene Id:403740Protein Name:prostaglandin-H2 D-isomeraseSwissprot Id:Q9XS65Protein Accession #:NP_001003131.1Nucleotide Accession #:NM_001003131.1Tissue Tool:Find tissues and cell lines supported by DNA array analysis to express PTGDS. RNA Seq:Find tissues and cell lines supported by RNA-seq analysis to express PTGDS. 我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物，这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌，哺乳动物细胞和昆虫细胞中表达，并作为整体裂解物提供。
