Predicted Species Reactivity:BovineApplication:ELISAKit Detection Method:Colorimetric, OD450 nmKit Principle:Aviva Systems Biology BGN ELISA Kit (Bovine) (OKEH07663) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BGN has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BGN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BGN captured in well.Kit Recovery:Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 87% (n = 20)Kit Reproducibility:Mean Intra-assay CV%: <=8.1% (n="">Mean Inter-assay CV%: <=10.5% (n="">Kit Component:| Component | Amount |
|---|
| BGN Microplate | 96 Wells (12 x 8 Well strips) |
| BGN Lyophilized Standard | 2 |
| 100X Biotinylated BGN Detector Antibody | 1 x 120 uL |
| 100X Avidin-HRP Conjugate | 1 x 120 uL |
| Sample Diluent | 1 x 20 mL |
| Detector Antibody Diluent | 1 x 12 mL |
| Conjugate Diluent | 1 x 12 mL |
| 25X Wash Buffer | 1 x 30 mL |
| TMB Substrate | 1 x 10 mL |
| Stop Solution | 1 x 10 mL |
Additional Information:Function: May be involved in collagen fiber assembly.:::Subcellular Location: Secreted Extracellular space Extracellular matrix:::Unigene: Bt.5351SMR: P21809String: 9913.ENSBTAP00000044462:::KEGG: bta:280733Pfam: PF13855:::KEGG: bta:280733Pfam: PF13855Reconstitution and Storage:Store as indicated in product manual.Datasheets/Manuals:Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.Specificity:Natural and recombinant Bovine BiglycanAssay Info:Assay Methodology: Quantitative Sandwich ELISAGene Symbol:BGNOfficial Gene Full Name:biglycanAlias Symbols:Bone/cartilage proteoglycan I, Leucine-rich PG I, PG-S1NCBI Gene Id:280733Protein Name:biglycanSwissprot Id:P21809Protein Accession #:NP_847888.2Nucleotide Accession #:NM_178318.4Tissue Tool:Find tissues and cell lines supported by DNA array analysis to express BGN. RNA Seq:Find tissues and cell lines supported by RNA-seq analysis to express BGN. 我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物，这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌，哺乳动物细胞和昆虫细胞中表达，并作为整体裂解物提供。
