Predicted Species Reactivity:BovineApplication:ELISAKit Detection Method:Colorimetric, OD450 nmKit Principle:Aviva Systems Biology IFI30 ELISA Kit (OKEH07716) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-IFI30 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated IFI30 and incubated. The IFI30 found in the sample or standards competes with the biotinylated IFI30 for limited binding sites on the immobilized anti-IFI30 antibody. Excess unbound biotinylated IFI30 and sample or standard IFI30 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated IFI30 captured in the well and inversely proportional to the amount of IFI30 which was contained in the sample or standard.Kit Range:0.312 - 20ng/mLKit Recovery:Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102% (n = 20)Kit Reproducibility:Mean Intra-assay CV%: <=7.0% (n="">Mean Inter-assay CV%: <=7.4% (n="">Kit Component:| Component | Amount |
|---|
| Anti-IFI30 Microplate | 96 Wells (12 x 8 Well strips) |
| IFI30 Lyophilized Standard | 2 x 100 ng |
| Sample Diluent | 1 x 20 mL |
| 100X IFI30-Biotin Complex | 1 x 60 uL |
| 100X Avidin-HRP Conjugate | 1 x 120 uL |
| Biotin Complex Diluent | 1 x 12 mL |
| Conjugate Diluent | 1 x 12 mL |
| 25X Wash Buffer | 1 x 30 mL |
| TMB Substrate | 1 x 10 mL |
| Stop Solution | 1 x 10 mL |
Additional Information:Function: Lysosomal thiol reductase that can reduce protein disulfide bonds. May facilitate the complete unfolding of proteins destined for lysosomal degradation. Plays an important role in antigen processing. Facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds. Facilitates also MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or crosspresentation.:::Subcellular Location: Secreted Lysosome:::Unigene: Bt.1548SMR: A6QPN6String: 9913.ENSBTAP00000045061:::KEGG: bta:615930Pfam: PF03227:::KEGG: bta:615930Pfam: PF03227Reconstitution and Storage:Store as indicated in product manual.Sensitivity:0.099ng/mLDatasheets/Manuals:Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.Specificity:Natural and recombinant Bovine Gamma-interferon-inducible lysosomal thiol reductaseAssay Info:Assay Methodology: Quantitative Competitive ELISAGene Symbol:IFI30Official Gene Full Name:IFI30 lysosomal thiol reductaseAlias Symbols:IFI30 proteinNCBI Gene Id:615930Protein Name:gamma-interferon-inducible lysosomal thiol reductaseSwissprot Id:A6QPN6Protein Accession #:NP_001094721.1Nucleotide Accession #:NM_001101251.2Tissue Tool:Find tissues and cell lines supported by DNA array analysis to express IFI30. RNA Seq:Find tissues and cell lines supported by RNA-seq analysis to express IFI30. 我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物，这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌，哺乳动物细胞和昆虫细胞中表达，并作为整体裂解物提供。
