Predicted Species Reactivity:BovineApplication:ELISAKit Detection Method:Colorimetric, OD450 nmKit Principle:Aviva Systems Biology P4HB ELISA Kit (Bovine) (OKEH07626) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for P4HB has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for P4HB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample P4HB captured in well.Kit Range:0.312 - 20ng/mLKit Recovery:Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 100% (n = 20)Kit Reproducibility:Mean Intra-assay CV%: <=2.7% (n="">Mean Inter-assay CV%: <=9.2% (n="">Kit Component:| Component | Amount |
|---|
| P4HB Microplate | 96 Wells (12 x 8 Well strips) |
| P4HB Lyophilized Standard | 2 |
| 100X Biotinylated P4HB Detector Antibody | 1 x 120 uL |
| 100X Avidin-HRP Conjugate | 1 x 120 uL |
| Sample Diluent | 1 x 20 mL |
| Detector Antibody Diluent | 1 x 12 mL |
| Conjugate Diluent | 1 x 12 mL |
| 25X Wash Buffer | 1 x 30 mL |
| TMB Substrate | 1 x 10 mL |
| Stop Solution | 1 x 10 mL |
Additional Information:Function: This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration.:::Subcellular Location: Endoplasmic reticulum Endoplasmic reticulum lumen Melanosome Cell membrane Peripheral membrane protein Highly abundant. In some cell types, seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources. Localizes near CD4-enriched regions on lymphoid cell surfaces. Colocalizes with MTTP in the endoplasmic reticulum.:::Unigene: Bt.106831SMR: P05307String: 9913.ENSBTAP00000007943:::KEGG: bta:281373Pfam: PF00085:::KEGG: bta:281373Pfam: PF00085Reconstitution and Storage:Store as indicated in product manual.Sensitivity:0.079ng/mLDatasheets/Manuals:Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.Specificity:Natural and recombinant Bovine Protein disulfide-isomeraseAssay Info:Assay Methodology: Quantitative Sandwich ELISAGene Symbol:P4HBOfficial Gene Full Name:prolyl 4-hydroxylase subunit betaAlias Symbols:PDINCBI Gene Id:281373Protein Name:protein disulfide-isomeraseSwissprot Id:P05307Protein Accession #:NP_776560.1Nucleotide Accession #:NM_174135.3Tissue Tool:Find tissues and cell lines supported by DNA array analysis to express P4HB. RNA Seq:Find tissues and cell lines supported by RNA-seq analysis to express P4HB. 我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物，这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌，哺乳动物细胞和昆虫细胞中表达，并作为整体裂解物提供。
