Product Format:Sterile filtered colorless solution.Host:Escherichia Coli.:::Product Introduction: RNMT is a widely expressed nuclear protein which is a member of the mRNA cap methyltransferase family. Cap-dependent mRNA translation requires the methylation of the mRNA guanosine cap by RNMT. RNMT catalyzes the transfer of a methyl group from AdoMet (S-adenosylmethionine) to the GpppN end of the growing mRNA at the N-7 position, thus producing AdoHyc (S-adenosylhomocysteine) and m7GpppN terminated RNA.Product Description: RNMT produced in E.Coli is a single, non-glycosylated polypeptide chain containing 496 amino acids (1-476 a.a.) and having a molecular mass of 57kDa. RNMT is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.Reconstitution and Storage:RNMT Human Recombinant although stable at 4C for 1 week, should be stored below -18C. Please prevent freeze thaw cycles.Purity:Greater than 90.0% as determined by SDS-PAGE.Peptide Sequence:MGSSHHHHHH SSGLVPRGSH MANSAKAEEY EKMSLEQAKA SVNSETESSF NINENTTASG TGLSEKTSVC RQVDIARKRK EFEDDLVKES SSCGKDTPSK KRKLDPEIVP EEKDCGDAEG NSKKRKRETE DVPKDKSSTG DGTQNKRKIA LEDVPEKQKN LEEGHSSTVA AHYNELQEVG LEKRSQSRIF YLRNFNNWMK SVLIGEFLEK VRQKKKRDIT VLDLGCGKGG DLLKWKKGRI NKLVCTDIAD VSVKQCQQRY EDMKNRRDSE YIFSAEFITA DSSKELLIDK FRDPQMCFDI CSCQFVCHYS FESYEQADMM LRNACERLSP GGYFIGTTPN SFELIRRLEA SETESFGNEI YTVKFQKKGD YPLFGCKYDF NLEGVVDVPE FLVYFPLLNE MAKKYNMKLV YKKTFLEFYE EKIKNNENKM LLKRMQALEP YPANESSKLV SEKVDDYEHA AKYMKNSQVR LPLGTLSKSE WEATSIYLVF AFEKQQ.Formulation:RNMT solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0) 2mM DTT, 20% glycerol and 100mM NaCl.Datasheets/Manuals:Printable datasheet for OOPA00127Purification:Greater than 90.0% as determined by SDS-PAGE.Gene Symbol:RNMTAlias Symbols:mRNA cap guanine-N7 methyltransferase, RG7MT1, mRNA (guanine-N(7)-)-methyltransferase, mRNA cap methyltransferase, hCMT1, hMet, hcm1p, RNMT, KIAA0398, MET, RG7MT1, hCMT1c, DKFZp686H1252. 我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物，这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌，哺乳动物细胞和昆虫细胞中表达，并作为整体裂解物提供。
