Predicted Species Reactivity:MouseProduct Format:Purified IgG - lyophilised Clonality:PolyclonalIsotype:Polyclonal IgGHost:RabbitApplication:WB:::Preservative Stabilisers: None presentAntiserum Preparation: Antisera to mouse TNF-alpha were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.:::Approx Protein Conc: IgG concentration 1.0mg/mlBuffer Solutions: Phosphate buffered saline pH7.4Reconstitution and Storage:Reconstitution: Reconstitute with 0.1ml distilled water. The addition of 0.09% sodium azide is recommended for long term storage. N.B. For functional studies do not add azide.Storage: Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.This product should be stored undiluted.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Replacement Item:This antibody may replace item sc-101425 from Santa Cruz Biotechnology.Immunogen:The immunogen for anti-Tnf antibody: recombinant mouse TNF-alphaPredicted Homology Based on Immunogen Sequence:MouseDatasheets/Manuals:Printable datasheet for anti-Tnf antibody - OASA08488Specificity:TNF ALPHAApplication Info:ELISA: 0.5ug/mlWestern Blotting: 0.1 - 0.2ug/mlFunctional Assays: 0.01 - 0.015ug/mlTarget Reference:1. Van Horssen, R. et al. (2006) TNF-alpha in cancer treatment: molecular insights, antitumor effects, and clinical utility. The Oncologist. 11: 397-408.Gene Symbol:TnfAlias Symbols:DIF, Tnfa, TNFSF2, Tnfsf1a, TNFalpha, MGC151434, TNF-alpha, TnfNCBI Gene Id:21926Protein Name:Tumor necrosis factorDescription of Target:RABBIT ANTI MOUSE TNF ALPHASwissprot Id:P06804Protein Accession #:NP_038721.1Protein Size (# AA):235Tissue Tool:Find tissues and cell lines supported by DNA array analysis to express Tnf. 
RNA Seq:Find tissues and cell lines supported by RNA-seq analysis to express Tnf. 
我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物，这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌，哺乳动物细胞和昆虫细胞中表达，并作为整体裂解物提供。
