Tested Species Reactivity:MousePredicted Species Reactivity:MouseProduct Format:Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.Clonality:PolyclonalHost:RabbitApplication:WBReconstitution and Storage:For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.Gene Symbol:NONOOfficial Gene Full Name:non-POU-domain-containing, octamer binding proteinNCBI Gene Id:53610Protein Name:non-POU domain-containing octamer-binding proteinSwissprot Id:Q99K48Protein Accession #:NP_001239447.1Nucleotide Accession #:NM_001252518.1Alias Symbols:nonA, NRB54, P54NRB, AA407051, AV149256Description of Target:DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double-stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site. Involved in pre-mRNA splicing, probably as a heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. Together with PSPC1, required for the formation of nuclear paraspeckles. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. NONO is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Important for the functional organization of GABAergic synapses. Plays a specific and important role in the regulation of synaptic RNAs and GPHN/gephyrin scaffold structure, through the regulation of GABRA2 transcript (PubMed:26571461).Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.Protein Size (# AA):473Molecular Weight:52 kDaPurification:Affinity purifiedTissue Tool:Find tissues and cell lines supported by DNA array analysis to express NONO. 
RNA Seq:Find tissues and cell lines supported by RNA-seq analysis to express NONO. 
Immunogen:The immunogen is a synthetic peptide directed towards the N terminal region of mouse NONOPeptide Sequence:Synthetic peptide located within the following region: QNHTPRKHHQHHHQQHHQQQQQQQQQQPPPPIPANGQQASSQNEGLTIDLConcentration:Batch dependent within range: 100 ul at 0.5 - 1 mg/mlBlocking Peptide:For anti-NONO (ARP89497_P050) antibody is Catalog # AAP89497Datasheets/Manuals:Printable datasheet for anti-NONO (ARP89497_P050) antibody 我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物，这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌，哺乳动物细胞和昆虫细胞中表达，并作为整体裂解物提供。
