Product Format:Liquid 100 mL, 107 cells/50 mg tissues by 1 mL lysis buffer:::Important Notes: Avoid freeze-thaw cycles, aliquot when receiving.Lysis process should be performed on ice.If salt precipitation occurs after long-term storage, set lysis buffer at 4°C, but it will dissolve after it"s placed at room temperature. This phenomenon is normal and would not affect the result of the experiment.Lysis buffer should be chosen according to the characteristics of samples and experimental purposes.Optimal experiment condition should be determined by end-user through pilot experiment.Contains sodium azide which may react with lead and copper plumbing to form explosive metallic azides. Flush with large volumes of water during disposal.Reconstitution and Storage:Store at 4°C for frequent use. Stored at -20°C for one year. Avoid repeated freeze-thaw cycles. In order to prevent protein degradation, it is recommended to add 1-2 mM PMSF into the lysis buffer before usage. Application Info:It"s applicable on most experiments, such as SDS-PAGE, Western Blotting (WB), Immunoprecipitation (IP), Co-IP, ELISA, etc. Datasheets/Manuals:Printable datasheet for OLCD00001Description of Target:Lysis buffer is used to lyse cells under non-denaturing buffer conditions. It consists of surfactant, a protease inhibitor, etc. It is mainly used for protein extraction from cells and tissues. This product is optimized from traditional lysis buffer. It"s suitable for lysis of membrane proteins, including surface proteins and receptotrs. which will help the release of proteins and do good for later extraciton, ELISA, Western Blot, etc. 我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物，这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌，哺乳动物细胞和昆虫细胞中表达，并作为整体裂解物提供。
