Product Format:Liquid:::Buffer: pH 7.2 + - 0.2Preservative: A non-mercury and azide-free active ingredient against microorganisms.Reconstitution and Storage:Store at 2-8 C. DO NOT FREEZE!Specificity:Specially formulated reagent to improve the stability and function of antigens/proteins bound to a solid phase. Use as supplied for very unstable proteins or for less labile antigens/proteins dilute up to 1:1 in your current blocker.Application Info:Performance was verified by testing with a labile autoimmune antigen. The antigen was coated with the stabilizer according to procedure, dried and incubated at 50C overnight. Product stabilization of the antigen is confirmed if the antigen maintains 80% mean activity when tested against panel sera. Recommended Protocol for Stabilizing and Blocking Immobilized Proteins: 1. After coating the surface with protein/antigen, wash once to remove excess and weakly adsorbed protein. 2. Before the protein begins to dry, coat the surface with the Coating Stabilizer and Blocking Buffer to completely cover the bound material. Allow to incubate at room temperature for 15 to 60 minutes. 3. Aspirate or drain the excess stabilizer from the surface. Do not wash the surface. 4. Dry the protein, preferably under vacuum. Recommended drying times are as follows: a. Two hours under vacuum (< 100="" micron).="" b.="" overnight="" in="" a="" humidity="" controlled="" chamber="" that="" registers="">< 15%="" humidity.="" 5.="" package="" the="" bound="" antigen/protein="" in="" a="" sealed="" airtight="" container="" with="" desiccant.="" the="" product="" is="" now="" stabilized="" for="" long-term="" storage="" at="" 2-8="" c.="" each="" laboratory="" should="" determine="" an="" optimum="" working="" titer="" for="" use="" in="" its="" particular="" application.="" other="" applications="" have="" not="" been="" tested="" but="" use="" in="" such="" assays="" should="" not="" necessarily="" be="">Datasheets/Manuals:Printable datasheet for OOMA00015Purification:Product is 0.2 um filtered.. 我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物,这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌,哺乳动物细胞和昆虫细胞中表达,并作为整体裂解物提供。