CryonaseCold-ActiveNucleaseisarecombinantendonucleaseoriginallyderivedfromapsychrophile,Shewanellasp.,andpurifiedfromE.coli.ThisendonucleaseenzymecancompletedigestionofalltypesofDNAandRNA(single-stranded,double-stranded,linearorcircularized)atcoldtemperaturesandeveninsamplesplacedonice.Asaresult,CryonaseCold-ActiveNucleaseisfrequentlyusedduringDNAdigestionofmixedsamples,suchasthosethatincludeheat-lABIleproteins.