ProductFormat:Liquid,1XPBS,5XEnhancerReagentApplication:WBReconstitutionandStorage:Diluteaccordingtouserinstructionsimmediatelypriortouse.Equilibratetoroomtemperaturepriortouse.Discardifanyprecipitatesarepresent.Storeat+4Cuponreceipt.Forfrequentusage,aliquotandstoreat+4C.Storeat-20Cfornolongerthan1year.ApplicationInfo:WesternBlotEnhnacerReagentisutilizedinWesternBlot(WB)applicationsasaprimaryandsecondaryantibodydilutionbuffer.Thisreagentwillincreasesensitivityandoverallsignal.ThereagentiscompatIBLewithPBSorTBSbuffersystemsandismosteffectivewhenusedinconjuctionwithanon-fatmilksolidblocker.Directionsforuse:1.Equilibrateallreagentstoambienttemperaturepriortouse.2.Stock5XWesternBlotEnhancerReagentisdiluted1:5withantibodydilutionbuffer(PBSorTBS).Mixonepart5XWesternBlotEnhancerReagentwith4partsantibodybuffer(PBSorTBS).3.Mixthe1XWesternBlotEnhancerReagentthoroughlyandgentlyfor30secondsbyinversiontoensurepropermixing.4.Immediatelypriortouse,diluteprimaryandsecondaryantibodytodesiredconcentrationintheworkingstrength1XWesternBlotEnhancerReagent.Notethatareductioninantibodyconcentrationisrecommendedtomitigateanynon-specificbinding.Optimalantibodyconcentrationmustbedeterminedbyuserforspecificapplications. Datasheets/Manuals:PrintabledatasheetforAOT00004 我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物，这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌，哺乳动物细胞和昆虫细胞中表达，并作为整体裂解物提供。
