Predicted Species Reactivity:BovineApplication:ELISAKit Detection Method:Colorimetric, OD450 nmKit Principle:Aviva Systems Biology PDGFA ELISA Kit (Bovine) (OKEH07302) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PDGFA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PDGFA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PDGFA captured in well.Kit Range:78 - 5000pg/mLKit Recovery:Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 107% (n = 20)Kit Reproducibility:Mean Intra-assay CV%: <=8.0% (n="">Mean Inter-assay CV%: <=8.2% (n="">Kit Component:| Component | Amount |
|---|
| PDGFA Microplate | 96 Wells (12 x 8 Well strips) |
| PDGFA Lyophilized Standard | 2 |
| 100X Biotinylated PDGFA Detector Antibody | 1 x 120 uL |
| 100X Avidin-HRP Conjugate | 1 x 120 uL |
| Sample Diluent | 1 x 20 mL |
| Detector Antibody Diluent | 1 x 12 mL |
| Conjugate Diluent | 1 x 12 mL |
| 25X Wash Buffer | 1 x 30 mL |
| TMB Substrate | 1 x 10 mL |
| Stop Solution | 1 x 10 mL |
Additional Information:Function: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB.:::Subcellular Location: Secreted Released by platelets upon wounding.:::Unigene: Bt.18780SMR: Q2KJ15String: 9913.ENSBTAP00000019323:::KEGG: bta:505908Pfam: PF00341:::KEGG: bta:505908Pfam: PF00341Reconstitution and Storage:Store as indicated in product manual.Sensitivity:39pg/mLDatasheets/Manuals:Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.Specificity:Natural and recombinant Bovine Platelet-derived growth factor subunit AAssay Info:Assay Methodology: Quantitative Sandwich ELISAGene Symbol:PDGFAOfficial Gene Full Name:platelet derived growth factor subunit AAlias Symbols:PDGF subunit A, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptideNCBI Gene Id:505908Protein Name:platelet-derived growth factor subunit ASwissprot Id:Q2KJ15Protein Accession #:NP_001068699.1Nucleotide Accession #:NM_001075231.1Tissue Tool:Find tissues and cell lines supported by DNA array analysis to express PDGFA. RNA Seq:Find tissues and cell lines supported by RNA-seq analysis to express PDGFA. 我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物，这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌，哺乳动物细胞和昆虫细胞中表达，并作为整体裂解物提供。
