Predicted Species Reactivity:PigApplication:ELISAKit Detection Method:Colorimetric, OD450 nmKit Principle:Aviva Systems Biology KIT ELISA Kit (Pig) (OKEH07317) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KIT has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for KIT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KIT captured in well.Kit Range:0.312 - 20ng/mLKit Recovery:Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 111% (n = 20)Kit Reproducibility:Mean Intra-assay CV%: <=5.9% (n="">Mean Inter-assay CV%: <=7.3% (n="">Kit Component:| Component | Amount |
|---|
| KIT Microplate | 96 Wells (12 x 8 Well strips) |
| KIT Lyophilized Standard | 2 |
| 100X Biotinylated KIT Detector Antibody | 1 x 120 uL |
| 100X Avidin-HRP Conjugate | 1 x 120 uL |
| Sample Diluent | 1 x 20 mL |
| Detector Antibody Diluent | 1 x 12 mL |
| Conjugate Diluent | 1 x 12 mL |
| 25X Wash Buffer | 1 x 30 mL |
| TMB Substrate | 1 x 10 mL |
| Stop Solution | 1 x 10 mL |
Additional Information:Function: Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.:::Subcellular Location: Cell membrane Single-pass type I membrane protein:::Unigene: Ssc.41222SMR: Q2HWD6String: 9823.ENSSSCP00000009430:::KEGG: ssc:396810Pfam: PF00047:::KEGG: ssc:396810Pfam: PF00047Reconstitution and Storage:Store as indicated in product manual.Sensitivity:0.15ng/mLDatasheets/Manuals:Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.Specificity:Natural and recombinant Pig Mast/stem cell growth factor receptor KitAssay Info:Assay Methodology: Quantitative Sandwich ELISAGene Symbol:KITAlias Symbols:MGFNCBI Gene Id:396810Protein Name:mast/stem cell growth factor receptor KitSwissprot Id:Q2HWD6Protein Accession #:NP_001037990.1Nucleotide Accession #:NM_001044525.1Tissue Tool:Find tissues and cell lines supported by DNA array analysis to express KIT. RNA Seq:Find tissues and cell lines supported by RNA-seq analysis to express KIT. 我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物，这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌，哺乳动物细胞和昆虫细胞中表达，并作为整体裂解物提供。
