Predicted Species Reactivity:DogApplication:ELISAKit Detection Method:Colorimetric, OD450 nmKit Linearity:| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 85-98% (n = 20) | 109-119% (n = 20) | 107-117% (n = 20) | 88-101% (n = 20) |
| EDTA plasma(n=5) | 99-109% (n = 20) | 90-99% (n = 20) | 98-106% (n = 20) | 105-115% (n = 20) |
| heparin plasma(n=5) | 93-102% (n = 20) | 97-108% (n = 20) | 108-118% (n = 20) | 108-119% (n = 20) |
Kit Principle:Aviva Systems Biology CCL2 ELISA Kit (Dog) (OKEH07277) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CCL2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CCL2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CCL2 captured in well.Kit Range:31.2 - 2000pg/mLKit Recovery:Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 81% (n = 20)Kit Reproducibility:Mean Intra-assay CV%: <=4.3% (n="">Mean Inter-assay CV%: <=7.3% (n="">Kit Component:| Component | Amount |
|---|
| CCL2 Microplate | 96 Wells (12 x 8 Well strips) |
| CCL2 Lyophilized Standard | 2 |
| 100X Biotinylated CCL2 Detector Antibody | 1 x 120 uL |
| 100X Avidin-HRP Conjugate | 1 x 120 uL |
| Sample Diluent | 1 x 20 mL |
| Detector Antibody Diluent | 1 x 12 mL |
| Conjugate Diluent | 1 x 12 mL |
| 25X Wash Buffer | 1 x 30 mL |
| TMB Substrate | 1 x 10 mL |
| Stop Solution | 1 x 10 mL |
Additional Information:Function: Chemotactic factor that attracts monocytes, but not neutrophils. Important factor in the course of the inflammatory reaction to reperfusion of the previously ischemic myocardium. May play a significant role in monocyte trafficking into the reperfused myocardium.:::Subcellular Location: SecretedReconstitution and Storage:Store as indicated in product manual.Sensitivity:15.6pg/mLDatasheets/Manuals:Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.Specificity:Natural and recombinant Dog C-C motif chemokine 2Assay Info:Assay Methodology: Quantitative Sandwich ELISAGene Symbol:CCL2Official Gene Full Name:C-C motif chemokine ligand 2Alias Symbols:Monocyte chemoattractant protein 1, Monocyte chemotactic protein 1, MCP-1, Small-inducible cytokine A2, MCP1, SCYA2NCBI Gene Id:403981Protein Name:C-C motif chemokine 2Swissprot Id:P52203Protein Accession #:NP_001003297.1Nucleotide Accession #:NM_001003297.1Tissue Tool:Find tissues and cell lines supported by DNA array analysis to express CCL2. RNA Seq:Find tissues and cell lines supported by RNA-seq analysis to express CCL2. 我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物，这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌，哺乳动物细胞和昆虫细胞中表达，并作为整体裂解物提供。
