Predicted Species Reactivity:PigApplication:ELISAKit Detection Method:Colorimetric, OD450 nmKit Linearity:| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 98-110% (n = 20) | 83-93% (n = 20) | 104-113% (n = 20) | 104-113% (n = 20) |
| EDTA plasma(n=5) | 99-108% (n = 20) | 98-98% (n = 20) | 97-109% (n = 20) | 107-116% (n = 20) |
| heparin plasma(n=5) | 83-93% (n = 20) | 107-118% (n = 20) | 92-101% (n = 20) | 104-116% (n = 20) |
Kit Principle:Aviva Systems Biology IGFBP2 ELISA Kit (Pig) (OKEH07255) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IGFBP2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IGFBP2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IGFBP2 captured in well.Kit Range:0.312 - 20ng/mLKit Recovery:Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 84% (n = 20)Kit Reproducibility:Mean Intra-assay CV%: <=5.5% (n="">Mean Inter-assay CV%: <=12.3% (n="">Kit Component:| Component | Amount |
|---|
| IGFBP2 Microplate | 96 Wells (12 x 8 Well strips) |
| IGFBP2 Lyophilized Standard | 2 |
| 100X Biotinylated IGFBP2 Detector Antibody | 1 x 120 uL |
| 100X Avidin-HRP Conjugate | 1 x 120 uL |
| Sample Diluent | 1 x 20 mL |
| Detector Antibody Diluent | 1 x 12 mL |
| Conjugate Diluent | 1 x 12 mL |
| 25X Wash Buffer | 1 x 30 mL |
| TMB Substrate | 1 x 10 mL |
| Stop Solution | 1 x 10 mL |
Additional Information:Function: May have both growth-inhibiting and growth-promoting effects, depending on tissue type; increases IGF-induced DNA synthesis in the uterine epithelium. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.:::Subcellular Location: Secreted:::Unigene: Ssc.47SMR: P24853String: 9823.ENSSSCP00000026205:::KEGG: ssc:397064Pfam: PF00219:::KEGG: ssc:397064Pfam: PF00219Reconstitution and Storage:Store as indicated in product manual.Sensitivity:0.15ng/mLDatasheets/Manuals:Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.Specificity:Natural and recombinant Pig Insulin-like growth factor-binding protein 2Assay Info:Assay Methodology: Quantitative Sandwich ELISAGene Symbol:IGFBP2Alias Symbols:pIGFBP-2NCBI Gene Id:397064Protein Name:insulin-like growth factor-binding protein 2Swissprot Id:P24853Protein Accession #:NP_999168.1Nucleotide Accession #:NM_214003.1Tissue Tool:Find tissues and cell lines supported by DNA array analysis to express IGFBP2. RNA Seq:Find tissues and cell lines supported by RNA-seq analysis to express IGFBP2. 我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物，这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌，哺乳动物细胞和昆虫细胞中表达，并作为整体裂解物提供。
