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Scientific Background: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bim/BOD is a group of three splice variants, BimEL, BimL and BimS, with apparent molecular masses of ~23, 16, and 13 kDa, respectively.Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat (1,2). Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines (1,2).

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Certificate of Analysis: 2.5 ug/mL was sufficient for detection of Bim in 20ug of K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Reconstitution and Storage:

Store at -20C. Shipping in Blue Ice or 4C.

Replacement Item:

This antibody may replace item sc-11425 from Santa Cruz Biotechnology.

Immunogen:

Internal central amino acids of human Bim

Purification:

Affinity Purified

Concentration:

1mg/mL

Datasheets/Manuals:

Printable datasheet for OASE00384

Dilution:

1:400 (WB), 1:100 (ICC)

Target Reference:

1. O"Connor L, Strasser A, O"Reilly LA, et al. Bim: a novel member of the Bcl-2 family that promotes apoptosis. EMBO J. 1998; 17:384-395.2. Hsu SY, Lin P, and Hsueh AJ BOD (Bcl-2-related ovarian death gene) is an ovarian BH3 domain-containing proapoptotic Bcl-2 protein capable of dimerization with diverse antiapoptotic Bcl-2 members. Mol. Endocrinol. 1998; 12:1432-40.

Storage Buffer:

PBS with 0.02% sodium azide

Gene Symbol:

BCL2L11

Official Gene Full Name:

BCL2-like 11 (apoptosis facilitator)

NCBI Gene Id:

10018

Swissprot Id:

O43521

Protein Accession #:

NP_619527.1