Predicted Species Reactivity:Human, MouseProduct Format:LiquidClonality:PolyclonalIsotype:IgGHost:RabbitApplication:IHC-P, ICC, WBReconstitution and Storage:Long term: -20C; Short term: +4C. Avoid repeat freeze-thaw cycles.Replacement Item:This antibody may replace item sc-112240 from Santa Cruz Biotechnology.Immunogen:NR4A1 / NUR77 antibody was raised against synthetic peptide from human NR4A1 / NUR77.Purification:Protein G purifiedConcentration:0.5 mg/mlDatasheets/Manuals:Printable datasheet for OALA01755Specificity:Nak1.Epitope:aa251-266Warning:For research use only.Application Info:IHC - Paraffin (20 - 40 ug/ml), ICC, Western blot (1:500 - 1:1000)Application Data:Immunohistochemistry: OALA01755 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for OALA01755 was determined to be 20-40 ug/ml.Storage Buffer:PBS, 0.05% BSA, 0.05% sodium azide.Gene Symbol:NR4A1Alias Symbols:NR4A1, GFRP1, Early response protein NAK1, HMR, NGFIB, Orphan nuclear receptor TR3, Orphan receptor tr3, Steroid receptor TR3, TR3, NAK1, NGFI-B, NP10, Receptor NGFIB, Testicular receptor 3, TR3 orphan receptor, N10, NAK-1, NUR77, OrphanNCBI Gene Id:3164Swissprot Id:P22736Protein Accession #:NP_002126.2Nucleotide Accession #:NM_002135Tissue Tool:Find tissues and cell lines supported by DNA array analysis to express NR4A1. RNA Seq:Find tissues and cell lines supported by RNA-seq analysis to express NR4A1. 我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物,这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌,哺乳动物细胞和昆虫细胞中表达,并作为整体裂解物提供。