Predicted Species Reactivity:Pig, Human, Rat, ChickenProduct Format:LiquidClonality:MonoclonalIsotype:IgG1Host:MouseApplication:IHC-P, IF, WB, IPReconstitution and Storage:Long term: Add glycerol (40-50%) -20C; Short term: +4CImmunogen:GFAP antibody was raised against porcine spinal cord. Cellular Localization: Cytoplasmic.Purification:Protein G purifiedConcentration:1 mg/mlDatasheets/Manuals:Printable datasheet for OALA02650Specificity:neural tumors such as neuroblastomas Schwannomas, as well as extra-CNS tumors are not labeled. Species cross-reactivity: human, and chicken.Warning:For research use only.Application Info:IHC - Paraffin (5 ug/ml), Immunofluorescence, Western blot (1 - 2 ug/ml), ImmunoprecipitationApplication Data:Immunohistochemistry: OALA02650 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for OALA02650 was determined to be 5 ug/ml. Immunoprecipitation (Native and denatured): 2 ug/mg protein lysate (Use Protein G). Western Blot: 1-2 ug/ml for 2 hrs at RT. Positive control: Astrocytoma, IMR5 and brain cells.Storage Buffer:10 mM PBS, pH 7.4.Gene Symbol:GFAPAlias Symbols:GFAPNCBI Gene Id:2670Swissprot Id:P14136Protein Accession #:NP_002046.1Nucleotide Accession #:NM_002055Tissue Tool:Find tissues and cell lines supported by DNA array analysis to express GFAP. RNA Seq:Find tissues and cell lines supported by RNA-seq analysis to express GFAP. 我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物，这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌，哺乳动物细胞和昆虫细胞中表达，并作为整体裂解物提供。
