Predicted Species Reactivity:HumanProduct Format:LiquidClonality:PolyclonalHost:RabbitApplication:IHC-P, WBReconstitution and Storage:Short term 4C, long term aliquot and store at -20C, avoid freeze thaw cycles. Store undiluted.Replacement Item:This antibody may replace item sc-293425 from Santa Cruz Biotechnology.Immunogen:B7-H1 / PD-L1 / CD274 antibody was raised against synthetic peptide from human CD274.Purification:Immunoaffinity purifiedConcentration:1 mg/mlDatasheets/Manuals:Printable datasheet for OALA04451Specificity:PDL-1.Epitope:InternalWarning:For research use only.Application Info:IHC - Paraffin (5 ug/ml), Western blotApplication Data:Immunohistochemistry: OALA04451 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for OALA04451 was determined to be 5 ug/ml.Storage Buffer:PBS, 0.02% sodium azide.Gene Symbol:CD274Alias Symbols:CD274, B7-H, B7 homolog 1, B7-H1, B7H1, CD274 molecule, CD274 antigen, PDCD1 ligand 1, PDL1, PD-L1, PDCD1L1, PDCD1LG1, Programmed death ligand 1NCBI Gene Id:29126Swissprot Id:Q9NZQ7Protein Accession #:NP_054862.1Nucleotide Accession #:NM_014143Tissue Tool:Find tissues and cell lines supported by DNA array analysis to express CD274. RNA Seq:Find tissues and cell lines supported by RNA-seq analysis to express CD274. 我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物,这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌,哺乳动物细胞和昆虫细胞中表达,并作为整体裂解物提供。