Predicted Species Reactivity:Human, MouseProduct Format:LiquidClonality:PolyclonalIsotype:Rabbit IgGHost:RabbitApplication:WBAdditional Information:Function: May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin- dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D- CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.Reconstitution and Storage:Store at 2-8C for up to 6 months. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.Replacement Item:This antibody may replace item sc-20220-R from Santa Cruz Biotechnology.Immunogen:Phospho-p21 Cip1 (Thr145) Antibody detects endogenous levels of p21 Cip1 only when phosphorylated at Threonine 145Purification:Peptide affinity purified.Predicted Homology Based on Immunogen Sequence:Human, MouseConcentration:1 mg/mLDatasheets/Manuals:Printable datasheet for anti-CDKN1A (OAAB20548) antibodyPreservative:0.02% Sodium Azide and 50% glycerolTarget Post-Translational Modification:Phospho-Thr145Formulation:Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Target Reference:Harper J.W.,et al.Cell 75:805-816(1993).El-Deiry W.S.,et al.Cell 75:817-825(1993).Xiong Y.,et al.Nature 366:701-704(1993).Jiang H.,et al.Mol. Cell. Differ. 1:285-299(1993).Jiang H.,et al.Oncogene 10:1855-1864(1995).Gene Symbol:CDKN1AOfficial Gene Full Name:cyclin-dependent kinase inhibitor 1AAlias Symbols:P21, CIP1, SDI1, WAF1, CAP20, CDKN1, MDA-6, p21CIP1NCBI Gene Id:1026Description of Target:This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene.Swissprot Id:P38936Protein Accession #:NP_000380.1Nucleotide Accession #:NM_000389.4Molecular Weight:18 kDaTissue Tool:Find tissues and cell lines supported by DNA array analysis to express CDKN1A. 
RNA Seq:Find tissues and cell lines supported by RNA-seq analysis to express CDKN1A. 
我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物，这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌，哺乳动物细胞和昆虫细胞中表达，并作为整体裂解物提供。
