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蚂蚁淘在线 / 品牌中心 / NewEastBio品牌新闻

Anti-RAS (A146P) Mouse Monoclonal Antibody

作者： 时间：2024-11-10 点击量：

货号： 26478
抗体名： RAS(A146P)
抗体英文名： RAS(A146P) Antibody
靶点： H-Ras、K-Ras、N-Ras
浓度： 1 mg/ml
应用范围： ELISA、IF、IHC
宿主： Mouse
供应商： NewEastBio
数量： 30000
级别： 实验级
目录编号： 26478
抗原来源： Mouse
保质期： 两年
适应物种： 脊椎动物
标记物： FITC
克隆性： 是
保存条件： -20℃
形态： 液体
亚型： IgG
免疫原： A synthetic peptide from the internal region of Ras which includes the mutation of A146P, human origin.
规格： 100μl
Ras (A146P) [26478][100ul] 
 
Catalog Number:  26478
Gene Symbol:  H-Ras; K-Ras; N-Ras
Description:   Anti-RAS (A146P) Mouse Monoclonal Antibody
Background:   The A146P mutation of KRas results in an amino acid substitution at position 146, from an alanine to a threonine. KRAS encodes a protein that is a member of the small GTPase superfamily. Ras A146P mutation results in decreased GTPase activity and constitutive signaling. It can be found in many tumors, such as lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.
Immunogen:   A synthetic peptide from the internal region of Ras which includes the mutation of A146P, human origin.
Applications:   ELISA, IF, IHC
Recommended Dilutions:   ELISA: 1:1000-1:5000 IF: 1:50-1:100 IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes A146P mutant, but not wild-type RAS of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Immunofluorescence:  
Immunofluorescence of cells expressing KRas proteins with anti-KRas (A146P) antibody. HEK293T cells were transfected with pCDNA3-GFP-KRas (A146P) plasmid , pCDNA3-GFP-KRas (WT) plasmid, pCDNA3-GFP-KRas (A146V) plasmid or pCDNA3-GFP-KRas (A146T) plasmid, then fixed and stained with anti-KRas (A146P) monoclonal antibody (Cat. #26478).
Publications:
1.  DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model    Gene Therapy 21, 888-896 (October 2014)
2.  Are all RAS mutations the same? Coexisting KRAS and NRAS mutations in a caecal adenocarcinoma and contiguous tubulovillous adenoma    J Clin Pathol. 2015 Aug;68(8):657-60. doi: 10.1136
温馨提示：不可用于临床治疗。

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