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蚂蚁淘在线 / 品牌中心 / NewEastBio品牌新闻

Anti-CTNNB1(D32N) Mouse Monoclonal Antibody

作者： 时间：2024-11-10 点击量：

货号： 26303
抗体名： CTNNB1(D32N)抗体
抗体英文名： CTNNB1(D32N) antibody
靶点： Beta-catenin, CTNNB, Catenin beta-1
浓度： 1mg/ml
应用范围： ELISA, WB, IHC
宿主： Mouse
供应商： NewEastBio
数量： 30000
级别： 实验级
目录编号： 26306
抗原来源： Mouse
保质期： 两年
适应物种： 脊椎动物
标记物： FITC
克隆性： 是
保存条件： -20℃
形态： 液体
亚型： IgG
免疫原： A synthetic peptide from the internal region of CTNNB1 which includes the mutation of D32N, human origin
规格： 100μl
CTNNB1 (D32N) [26303][100ul] 
Catalog Number:  26303
Gene Symbol:  Beta-catenin, CTNNB, Catenin beta-1
Description:   Anti-CTNNB1(D32N) Mouse Monoclonal Antibody
Background:   CTNNB1 protein is a dual function protein. It is a subunit of a complex of proteins that from adherens junctions, which are important for the establishment and maintenance of epithelial cell layers by regulating cell growth and adhesion between adjacent cells. CTNNB1 protein also pulls double duty as an intracellular signal transducer in the Wnt signaling pathway. Mutations of CTNNB1 have been implicated in the pathogenesis of several cancers.
Immunogen:   A synthetic peptide from the internal region of CTNNB1 which includes the mutation of D32N, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes D32N mutant, but not wild-type CTNNB1 of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant CTNNB1 (D32N) and wildtype proteins. Purified His-tagged CTNNB1 (D32N) protein (lane2) and corresponding wild-type protein (lane1) were blotted with anti-CTNNB1 (D32N) monoclonal antibody (Cat. #26303).
Immunofluorescence:  
Immunofluorescence of cells expressing CTNNB1 proteins with anti-CTNNB1 (D32N) antibody. HEK293T cells were transfected with pCDNA3-GFP-CTNNB1 (WT) plasmid (left column) or pCDNA3-GFP-CTNNB1 (D32N) plasmid (right column), then fixed and stained with anti-CTNNB1 (D32N) monoclonal antibody (Cat. #26303).

 

温馨提示：不可用于临床治疗。

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