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商品描述
| CatalogNumber: 10104 | |||||||||
| ProductName: RhoAProtein | |||||||||
| Synonyms: Rashomologgenefamily,memberA,ARHA,ARH12,RHO12,RHOH12 | |||||||||
| Source: Human,recombinantfulllength,His6-tag | |||||||||
| ExpressionHost: E.coli | |||||||||
| MolecularWeight: 22kDa | |||||||||
| Purity: >96%bySDS-PAGE | |||||||||
| Introduction: SmallGTPasesareasuper-familyofcellularsignalingregulators.RhoAbelongstotheRhosub-familyofGTPases.RhoproteinsplaycriticalrolesinmanyactinCytoskeleton-dependentprocessesincludingplateletaggregation,cellmotility,contraction,andcytokinesis.ItregulatestheformationofstressfibersandfocaladhesionsinfibroblastsandCa2+sensitivityofsmoothmusclecontraction. | |||||||||
| AminoAcidSequence (1-193) | |||||||||
MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYD RLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKM KQEPVKPEEGRDMANRIGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKKSGCLVL| Properties | PhysicalAppearance(form): Dissolvedin20mMTris-HCl,pH8.0,150mMNaCl. | PhysicalAppearance(form): Whiteorclear | Concentration: 1mg/mL | Storage: -80°C | PreparationInstructions: Centrifugethevialbeforeopenthecapandreconstituteinwater.Addingof10mMβ-mercaptoethanolor1mMDTTintothesolutiontoprotecttheproteinisrecommendedandusingofnon-ionicdetergentssuchasn-Dodecylβ-D-maltoside(DoDM)orpolyethylenedetergents(e.g.C12E10)alsohelptostABIlizetheprotein.Avoidrepeatedfreezingandthawingafterreconstitution.ThepurityofHis-taggedRhoAwasdeterminedbySDS-PAGEandCoomassieBrilliantBlueStaining. | References: 1.Canman,J.C.etal.,Science322:1543-1546,2008.2.Guilluy,C.etal.,NatureMed.16:183-190,2010.3.Machacek,M.etal.,Nature461:99-103,20094.Nakamura,M.etal.,Invest.Ophthal.Vis.Sci.42:941-947,2001.5.Rao,P.V.etal.,Invest.Ophthal.Vis.Sci.42:1029-1037,2001.6.Valderrama,F.etal.,Science311:377-381,2006.7.Wang,H.-R.etal.,Science302:1775-1779,2003.8.Wu,K.Y.etal.,Nature436:1020-1024,2005.9.Yoshida,S.etal.,Science313:108-111,2006. | Datasheet: |  |
