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商品描述
| CatalogNumber: 10152 | |||||||||
| ProductName: Arl3Protein | |||||||||
| Synonyms: ADP-ribosylationfactor-like3,ARFL3 | |||||||||
| Source: Human,recombinantfulllength,His6-tag | |||||||||
| ExpressionHost: E.coli | |||||||||
| MolecularWeight: 20kDa | |||||||||
| Purity: >95%bySDS-PAGE | |||||||||
| Introduction: Arl3belongstothememberoftheArffamilyofregulatoryGTPases,withintheRassuperfamilyofGTPases.Arl3isalsoamemberoftheADP-ribosylationfactorfamilyofGTP-bindingproteins.Arl3bindsguaninenucleotidesbutlacksADP-ribosylationfactoractivity. | |||||||||
| AminoAcidSequence (1-182) | |||||||||
MGLLSILRKLKSAPDQEVRILLLGLDNAGKTTLLKQLASEDISHITPTQGFNIKSVQSQGFKLNVWD IGGQRKIRPYWKNYFENTDILIYVIDSADRKRFEETGQELAELLEEEKLSCVPVLIFANKQDLLTAA PASEIAEGLNLHTIRDRVWQIQSCSALTGEGVQDGMNWVCKNVNAKKK| Properties | PhysicalAppearance(form): Dissolvedin20mMTris-HCl,pH8.0,150mMNaCl. | PhysicalAppearance(form): Whiteorclear | Concentration: 1mg/mL | Storage: -80°C | PreparationInstructions: Centrifugethevialbeforeopenthecapandreconstituteinwater.Addingof10mMβ-mercaptoethanolor1mMDTTintothesolutiontoprotecttheproteinisrecommendedandusingofnon-ionicdetergentssuchasn-Dodecylβ-D-maltoside(DoDM)orpolyethylenedetergents(e.g.C12E10)alsohelptostABIlizetheprotein.Avoidrepeatedfreezingandthawingafterreconstitution.ThepurityofHis-taggedArl3wasdeterminedbySDS-PAGEandCoomassieBrilliantBlueStaining. | References: 1.Cavenagh,M.M.etal.,J.Biol.Chem.269:18937-18942,1994.2.Grayson,C.etal.,Hum.Molec.Genet.11:3065-3074,2002.3.Kim,H.-S.Cytogenet.CellGenet.83:246-only,1998.4.Schrick,J.JAm.J.Path.168:1288-1298,2006.5.Veltel,S.etal.,NatureStruct.Molec.Biol.15:373-380,2008. | Datasheet: |  |
