ProductHighlight

• BasedontheuniquemousemonoclonalAnti-cAMPantibodyItistheonlyEIAkitonthemarketwithmonoclonalanti-cAMPantibody(allotherkitsarebasedonpolyclonalanti-cAMPantibodies). 
• Nomorehazardouschemical(aceticanhydride)foryourcAMPassayThemonoclonalAnti-cAMPantibodyhassimilaraffinitiesfornon-acetylatedcAMPandacetylatedcAMP.Thusacetylationwiththeaceticanhydrideispermanentlyeliminatedfromtheassay. 
• SupersensitivityandselectivityThemonoclonalAnti-cAMPantibodydisplays>10⁸foldofselectivityovercGMP,ATP,andothernucleosideanalogues.TheAnti-cAMPEIAKitprovidessignificantlyimprovedsensitivityandselectivityoverotherkitsbasedonpolyclonalanti-cAMPantibodiesonthemarket. 
• Shortestassaytimeandhigh-throughputformatTheAnti-cAMPEIAKitisthebestchoicefordrugscreenings.

ProductDescription

Adenosine3",5"-cyclicmonophosphate(cyclicAMP;cAMP)modulatesvariousphysiologicalfunctionssuchascardiovascularBIOLOGy,learningandmemory,olfaction,immuneresponse,asthmaandkidneyfunction(1,2).cAMPisproducedfromATPbyadenylylcyclasesandisdegradedbyphosphodiesterases.StimulationofadenylylcyclasesorinhibitionofphosphodiesterasescanincreasecellularcAMPconcentrations.Blockersofadenylylcyclase-activatingreceptorsandinhibitorsofthecAMP-specificphosphodiesterasesareusedfortreatinghumandiseases.Forexample,blockingagentsforcAMP-increasingbeta-adrenergicreceptors(beta-blockers)areusedfortreatingabnormalheartrhythms,highbloodpressure(hypertension),myocardialinfarctionandheartfailure.InhibitorsofcAMPspecificphosphodiesterasetypes2and4arebeingtestedforcognitionenhancement.

ToscreenforinhibitorsorstimulatorsofcellularcAMPlevels,itisessentialtohaveasensitive,selectiveandreproducIBLemethodtomeasurethecAMPconcentrations.Thisisespeciallytruefortheinitialscreeningsgiventhepossibleweakereffectsoflargerpoolsofcompounds.

CurrentlyavailableotherELISAkitsmeasuringcAMPlevelsarebasedonthenon-affinity-purifiedpolyclonalanti-cAMPantibody.Despitetheclaimedselectivity,thesepolyclonalanti-cAMPantibodiesdisplaycertaincross-reactivitywithATP.GiventhatATPisthesubstrateforthecAMPproduction,itisverydesirabletohaveanantibodywithhighspecificitytowardscAMPoverATP.

NewEastBiosciencescAMPELISAkitisbasedontheuniquemousemonoclonalanti-cAMPantibody.Thismonoclonalanti-cAMPantibodydisplays>10⁸foldofselectivityoverATP,cGMP,andothernucleosideanalogues.NewEastBiosciencescAMPELISAkitprovidessignificantlyimprovedsensitivityandselectivityoverotherkitsbasedonpolyclonalanti-cAMPantibodies.Ourmonoclonalanti-cAMPantibody-basedELISAkitalsoavoidsthebatch-to-batchvariationsassociatedwithpolyclonalantibodyproductionsfromanimals,thusprovidingthereproducibilityinthelongrun.

FurThermore,whilepolyclonalanti-cAMPantibodiesusedinotherELISAkitshavehigheraffinityforacetylatedcAMPthannon-acetylatedcAMP,NewEastBiosciencesmonoclonalanti-cAMPantibodyhassimilaraffinitiestonon-acetylatedandacetylatedcAMPmolecules.Therefore,acetylationtreatmentsofsamplesandstandardsarenotneededinNewEastBiosciencescAMPELISAkit.Thissignificantlyreducesthetimefortheassay.Theavoidanceoforganicreagentsusedintheacetylationprocessprovidesasafeandhealthyworkenvironment.

PrincipleOutline

NewEastBiosciencescAMPELISAKitisacompetitiveimmunoassaytomeasurethecAMPlevels,eitherfromcellextractsorfrominvitroadenylylcyclaseassays.Briefly,multi-wellplatesarecoatedwithgoat-anti-mouseserum.cAMPincellextractsorininvitroadenylylcyclaseassayswillcompetitivelybindtothemonoclonalanti-cAMPantibodyinthepresenceoffixedamountsofcAMP-conjugatedhorse-rADIshperoxidaseoralkalinephosphatase.KnownamountsofcAMPareusedasstandardstogeneratethecalculationcurve.Afterashortincubation,theexcessreagentsarewashedawayandsubstrateisadded.Themultiwellplatesarethenreadonamicroplatereaderat450nmor405nm.TheintensityoftheyellowcolorisinverselyproportionaltotheconcentrationofcAMPinsamples.ThemeasuredopticaldensityisusedtocalculatetheconcentrationofcAMPinsamplesbasedonthecurvefromthecAMPstandards.

   FAQs

   ProductManual

   AssayLayoutSheet NewEastBio我们的理念是Our Vision以信誉求发展，以质量树品牌，为科研服务，为生命尽责

• 

  资质认证

  获得国家资质，权威认证！

• 

  全国联保

  全国联保，官方无忧售后

• 

  正规发票

  正规发票，放心购买

• 

  签订合同

  签订合同，保障您的权益

购物指南

购物流程

注册登录

账户管理

订单查看

常见问题

FAQS

蚁豆兑换

物流说明

评论规则

发票说明

价格说明

支付说明

服务协议

版权声明

诚信保障

免责申明

销售协议

隐私条款

法律申明

售后服务

退换货政策

工作时间

退款说明

售后支持

售后申请表模板列表

关于我们

公司简介

联系我们

资质证书

人才招聘

商务合作

万众创业

网址导航

蚂蚁淘商城

科研狗

苏州蚂蚁淘

生物信息网

现货促销


微信公众号

ICL中国 | Polysciences经销商 | apmpolymers公司 | Biotium中国 | scandinavianformulas | Lumiprobe活性荧光染料试剂 | Alsachim授权 | 蚂蚁淘 | 南京信帆生物技术有限公司

copyright©2005-2021 蚂蚁淘在线版权所有　苏州蚂蚁淘生物科技有限公司 www.assay-online.com ICP号：苏ICP备17049038号-16

/**/

Publications:

1.  Heterogeneityinrelaxationofdifferentsizedporcinecoronaryarteriestonitrovasodilators:roleofPKGandMYPT1    PflugersArch.2012Feb;463(2):257-68

2.  Antiarrhythmiceffectofprolongedmorphineexposureisaccompaniedbyalteredmyocardialadenylylcyclasesignalinginrats    PharmacolRep.2012;64(2):351-9

3.  UncouplingofM1muscarinicreceptor/G-proteininteractionbyamyloidbeta(1-42)    Neuropharmacology.2013Apr;67:272-83

4.  SubendocardialIncreaseinReactiveOxygenSpeciesProductionAffectsRegionalContractileFunctioninIschemicHeartFailure    AntioxidRedoxSignal.2013Mar20;18(9):1009-20

5.  Heterogeneityinrelaxationofdifferentsizedporcinecoronaryarteriestonitrovasodilators:roleofPKGandMYPT1    PflugersArch.2012Feb;463(2):257-68

6.  Structuralbasisofanthraxedemafactorneutralizationbyaneutralizingantibody    BiochemicalandBiophysicalResearchCommunicationsVolume417,Issue1,6January2012,Pages324–329

7.  TransgenicrescueofdefectiveCd36enhancesmyocardialadenylylcyclasesignalinginspontaneouslyhypertensiverats    PflügersArchiv-EuropeanJournalofPhysiologyOctober2013,Volume465,Issue10,pp1477-1486

8.  OpposingHDAC4nuclearfluxesduetophosphorylationbyβadrenergicactivatedPKAorbyactivityorEpacactivatedCaMKIIinskeletalmusclefibres    TheJournalofPhysiologyVolume591,Issue14,pages3605–3623,July2013

9.  SexisamajordeterminantofneuronaldysfunctioninNeurofibromatosisType1    AnnalsofNeurologyVolume75,Issue2,pages309–316,February2014

10.  cAMP–PKAinhibitionofSK3channelreducedbothCa2+entryandcancercellmigrationbyregulationofSK3–Orai1complex    PflügersArchiv-EuropeanJournalofPhysiologyOctober2014,Volume466,Issue10,pp1921-1932

11.  Immunomodulatingeffectsofcasein-derivedpeptideQEPVLandQEPVonlymphocytesinvitroandinvivo    FoodFunct.,2014,5,2061-2069

12.  AgoNIST-DependentAnd-IndependentDopamine-1-LikeReceptorSignallingDifferentlyRegulatesDownstreamEffectors    FEBSJournalVolume281,Issue21,pages4792–4804,November2014

13.  Alterationofvascularreactivityinheartfailure:Roleofphosphodiesterasestype3and4    BritishJournalofPharmacologyVolume171,Issue23,pages5361–5375,December2014

14.  Ischemia/Reperfusion-InducedCHOPExpressionPromotesApoptosisandImpairsRenalFunctionRecovery:TheRoleofAcidosisandGPR4    PLoSOne.2014Oct24;9(10):e110944

15.  Comparativestudyofsomatostatin-humanserumalbuminfusionproteinsandnaturalsomatostatinonreceptorbinding,internalizationandactivation    PLoSOne.2014Feb27;9(2):e89932