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蚂蚁淘在线 / 品牌中心 / NewEastBio / NewEast/Monoclonal Anti-cGMP EIA Kit without Acetylation [80104][5x96 well]/80104

NewEast/Monoclonal Anti-cGMP EIA Kit without Acetylation [80104][5x96 well]/80104

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￥31900.00

货号：80104

浏览量：127

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ProductHighlight

BasedontheuniquemousemonoclonalAnti-cGMPantibodyItistheonlyEIAkitonthemarketwithmonoclonalanti-cGMPantibody(allotherkitsarebasedonpolyclonalanti-cGMPantibodies).
Nomorehazardouschemical(aceticanhydride)foryourcGMPassayThemonoclonalAnti-cGMPantibodyhassimilaraffinitiesfornon-acetylatedcGMPandacetylatedcGMP.Thusacetylationwiththeaceticanhydrideispermanentlyeliminatedfromtheassay.
SupersensitivityandselectivityThemonoclonalAnti-cGMPantibodydisplays>10⁸foldofselectivityovercAMP,GTP,andothernucleosideanalogues.TheAnti-cGMPEIAKitprovidessignificantlyimprovedsensitivityandselectivityoverotherkitsbasedonpolyclonalanti-cGMPantibodiesonthemarket.
Shortestassaytimeandhigh-throughputformatTheAnti-cGMPEIAKitisthebestchoicefordrugscreenings.

ProductDescription

Guanosine3",5"-cyclicmonophosphate(cyclicGMP;cGMP)playscriticalregulatoryrolesinmanyphysiologicalprocesses.cGMPisproducedfromGTPbyguanylylcyclasesandisdegradedbyphosphodiesterases.StimulationofguanylylcyclasesorinhibitionofphosphodiesterasescanincreasecellularcGMPconcentrations.InhibitorsofthecGMP-specificphosphodiesterasesareusedfortreatinghumandiseases.Forexample,inhibitorsofcGMPspecificphosphodiesterasetype5(suchasViagraandCialis)areusedfortreatingerectiledysfunction.

Toscreenforinhibitorsofphosphodiesterasesorstimulatorsofguanylylcyclases,itisessentialtohaveafast,sensitive,selectiveandreproducIBLemethodtomeasurethecGMPconcentrations.Thisisespeciallytruefortheinitialscreeningsgiventhepossibleweakereffectsoflargerpoolsofcompounds.

CurrentlyavailableotherELISAkitsmeasuringcGMPlevelsarebasedonthenon-affinity-purifiedpolyclonalanti-cGMPantibody.Despitetheclaimedselectivity,thesepolyclonalanti-cGMPantibodiesdisplaycertaincross-reactivitywithcAMPorGTP.Inmostcelltypes,cGMPispresentatlevels~100foldlowerthancAMP.

NewEastBiosciencescGMPELISAkitisbasedontheuniquemousemonoclonalanti-cGMPantibody.Thismonoclonalanti-cGMPantibodydisplays>108foldofselectivityovercAMP,GTP,andothernucleosideanalogues.NewEastBiosciencescGMPELISAkitprovidessignificantlyimprovedsensitivityandselectivityoverotherkitsbasedonpolyclonalanti-cGMPantibodies.Ourmonoclonalanti-cGMPantibody-basedELISAkitalsoavoidsthebatch-to-batchvariationsassociatedwithpolyclonalantibodyproductionsfromanimals,thusprovidingthereproducibilityinthelongrun.

FurThermore,whilepolyclonalanti-cGMPantibodiesusedinotherELISAkitshavehigheraffinityforacetylatedcGMPthannon-acetylatedcGMP,NewEastBiosciencesmonoclonalanti-cGMPantibodyhassimilaraffinitiestonon-acetylatedandacetylatedcGMPmolecules.Therefore,acetylationtreatmentsofsamplesandstandardsarenotneededinNewEastBiosciencescGMPELISAkit.Thissignificantlyreducesthetimefortheassay.Theavoidanceoforganicreagentsusedintheacetylationprocessprovidesasafeandhealthyworkenvironment.

PrincipleOutline

NewEastBiosciencescGMPELISAKitisacompetitiveimmunoassaytomeasurethecGMPlevels,eitherfromcellextractsorfrominvitroguanylylcyclaseassays.Briefly,multi-wellplatesarecoatedwithgoat-anti-mouseserum.cGMPincellextractsorininvitroguanylylcyclaseassayswillcompetitivelybindtothemonoclonalanti-cGMPantibodyinthepresenceoffixedamountsofcGMP-conjugatedhorse-rADIshperoxidase.KnownamountsofcGMPareusedasstandardstogeneratethecalculationcurve.Afterashortincubation,theexcessreagentsarewashedawayandsubstrateisadded.Themultiwellplatesarethenreadonamicroplatereaderat450nm.TheintensityoftheyellowcolorisinverselyproportionaltotheconcentrationofcGMPinsamples.ThemeasuredopticaldensityisusedtocalculatetheconcentrationofcGMPinsamplesbasedonthecurvefromthecGMPstandards.

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Publications:

1. Sulfhydryl-dependentdimerizationofsolubleguanylylcyclasemodulatestherelaxationofporcinepulmonaryarteriestonitricoxide PflugersArch.2013Feb;465(2):333-41

2. Pressure-overload-inducedsubcellularrelocalization/oxidationofsolubleguanylylcyclaseintheheartmodulatesenzymestimulation CircRes.2012Jan20;110(2):295-303

3. Natriureticpeptidesblocksynaptictransmissionbyactivatingphosphodiesterase2AandreducingpresynapticPKAactivity ProcNatlAcadSciUSA.2012Oct23;109(43):17681-6

4. Roleofsulfhydryl-dependentdimerizationofsolubleguanylylcyclaseinrelaxationofporcinecoronaryarterytonitricoxide CardiovascRes.2011Jun1;90(3):565-72

5. Molt-inhibitinghormonefromChinesemittencrab(Eriocheirsinensis):Cloning,tissueexpressionandeffectsofrecombinantpeptideonecdysteroidsecretionofYOs GenCompEndocrinol.2011Sep15;173(3):467-74

6. InhibitionofStriatalSolubleGuanylylCyclase-cGMPSignalingReversesBasalGangliaDysfunctionandAkinesiainExperimentalParkinsonism PLoSOne.2011;6(11):e27187

7. Molecularanalysis,developmentalfunctionandheavymetal-inducedexpressionofABCC5inzebrafish CompBiochemPhysiolBBiochemMolBiol.2011Jan;158(1):46-55

8. FeedbackcontrolthroughcGMP-dependentproteinkinasecontributestodifferentialregulationandcompartmentationofcGMPinratcardiacmyocytes CircRes.2010Nov12;107(10):1232-40

9. TheSolubleGuanylateCyclaseActivatorBAY58-2667ProtectsagainstMorbidityandMortalityinEndotoxicShockbyRecouplingOrganSystems PLoSOne.2013Aug28;8(8):e72155

10. Stimulationofguanylylcyclase-Dbybicarbonate Biochemistry.2009May26;48(20):4417-22

11. Volumeoverloadinducesdifferentialspatiotemporalregulationofmyocardialsolubleguanylylcyclaseineccentrichypertrophyandheartfailure JournalofMolecularandCellularCardiologyVolume60,July2013,Pages72–83

12. DefiningSpecificityDeterminantsofCyclicGMP-MediatedGustatorySensoryTransductioninCaenorhaBDitiselegans genetics.113.152660v1194/4/885

13. Theroleofnitricoxidesignalinginfoodintake;insightsfromtheinnermitochondrialmembranepeptidase2mutantmice RedoxBIOLOGyVolume1,Issue1,2013,Pages498–507

14. CrossregulationbetweencGMP-dependentproteinkinaseandAktinvasodilatationofporcinepulmonaryartery JournalofCardiovascularPharmacology:November2014-Volume64-Issue5-p452-459

15. Geneticablationofsolutecarrierfamily7a3aleadstohepaticsteatosisinzebrafishduringfasting HepatologyVolume60,Issue6,pages1929–1941,December2014