ProductHighlight- BasedontheuniquemousemonoclonalAnti-cGMPantibodyItistheonlyEIAkitonthemarketwithmonoclonalanti-cGMPantibody(allotherkitsarebasedonpolyclonalanti-cGMPantibodies).
- Nomorehazardouschemical(aceticanhydride)foryourcGMPassayThemonoclonalAnti-cGMPantibodyhassimilaraffinitiesfornon-acetylatedcGMPandacetylatedcGMP.Thusacetylationwiththeaceticanhydrideispermanentlyeliminatedfromtheassay.
- SupersensitivityandselectivityThemonoclonalAnti-cGMPantibodydisplays>108foldofselectivityovercAMP,GTP,andothernucleosideanalogues.TheAnti-cGMPEIAKitprovidessignificantlyimprovedsensitivityandselectivityoverotherkitsbasedonpolyclonalanti-cGMPantibodiesonthemarket.
- Shortestassaytimeandhigh-throughputformatTheAnti-cGMPEIAKitisthebestchoicefordrugscreenings.
ProductDescriptionGuanosine3",5"-cyclicmonophosphate(cyclicGMP;cGMP)playscriticalregulatoryrolesinmanyphysiologicalprocesses.cGMPisproducedfromGTPbyguanylylcyclasesandisdegradedbyphosphodiesterases.StimulationofguanylylcyclasesorinhibitionofphosphodiesterasescanincreasecellularcGMPconcentrations.InhibitorsofthecGMP-specificphosphodiesterasesareusedfortreatinghumandiseases.Forexample,inhibitorsofcGMPspecificphosphodiesterasetype5(suchasViagraandCialis)areusedfortreatingerectiledysfunction. Toscreenforinhibitorsofphosphodiesterasesorstimulatorsofguanylylcyclases,itisessentialtohaveafast,sensitive,selectiveandreproducIBLemethodtomeasurethecGMPconcentrations.Thisisespeciallytruefortheinitialscreeningsgiventhepossibleweakereffectsoflargerpoolsofcompounds. CurrentlyavailableotherELISAkitsmeasuringcGMPlevelsarebasedonthenon-affinity-purifiedpolyclonalanti-cGMPantibody.Despitetheclaimedselectivity,thesepolyclonalanti-cGMPantibodiesdisplaycertaincross-reactivitywithcAMPorGTP.Inmostcelltypes,cGMPispresentatlevels~100foldlowerthancAMP. NewEastBiosciencescGMPELISAkitisbasedontheuniquemousemonoclonalanti-cGMPantibody.Thismonoclonalanti-cGMPantibodydisplays>108foldofselectivityovercAMP,GTP,andothernucleosideanalogues.NewEastBiosciencescGMPELISAkitprovidessignificantlyimprovedsensitivityandselectivityoverotherkitsbasedonpolyclonalanti-cGMPantibodies.Ourmonoclonalanti-cGMPantibody-basedELISAkitalsoavoidsthebatch-to-batchvariationsassociatedwithpolyclonalantibodyproductionsfromanimals,thusprovidingthereproducibilityinthelongrun. FurThermore,whilepolyclonalanti-cGMPantibodiesusedinotherELISAkitshavehigheraffinityforacetylatedcGMPthannon-acetylatedcGMP,NewEastBiosciencesmonoclonalanti-cGMPantibodyhassimilaraffinitiestonon-acetylatedandacetylatedcGMPmolecules.Therefore,acetylationtreatmentsofsamplesandstandardsarenotneededinNewEastBiosciencescGMPELISAkit.Thissignificantlyreducesthetimefortheassay.Theavoidanceoforganicreagentsusedintheacetylationprocessprovidesasafeandhealthyworkenvironment. PrincipleOutlineNewEastBiosciencescGMPELISAKitisacompetitiveimmunoassaytomeasurethecGMPlevels,eitherfromcellextractsorfrominvitroguanylylcyclaseassays.Briefly,multi-wellplatesarecoatedwithgoat-anti-mouseserum.cGMPincellextractsorininvitroguanylylcyclaseassayswillcompetitivelybindtothemonoclonalanti-cGMPantibodyinthepresenceoffixedamountsofcGMP-conjugatedhorse-rADIshperoxidase.KnownamountsofcGMPareusedasstandardstogeneratethecalculationcurve.Afterashortincubation,theexcessreagentsarewashedawayandsubstrateisadded.Themultiwellplatesarethenreadonamicroplatereaderat450nm.TheintensityoftheyellowcolorisinverselyproportionaltotheconcentrationofcGMPinsamples.ThemeasuredopticaldensityisusedtocalculatetheconcentrationofcGMPinsamplesbasedonthecurvefromthecGMPstandards.  FAQs
ProductManual AssayLayoutSheet Publications:| 1. Sulfhydryl-dependentdimerizationofsolubleguanylylcyclasemodulatestherelaxationofporcinepulmonaryarteriestonitricoxide PflugersArch.2013Feb;465(2):333-41 | | 2. Pressure-overload-inducedsubcellularrelocalization/oxidationofsolubleguanylylcyclaseintheheartmodulatesenzymestimulation CircRes.2012Jan20;110(2):295-303 | | 3. Natriureticpeptidesblocksynaptictransmissionbyactivatingphosphodiesterase2AandreducingpresynapticPKAactivity ProcNatlAcadSciUSA.2012Oct23;109(43):17681-6 | | 4. Roleofsulfhydryl-dependentdimerizationofsolubleguanylylcyclaseinrelaxationofporcinecoronaryarterytonitricoxide CardiovascRes.2011Jun1;90(3):565-72 | | 5. Molt-inhibitinghormonefromChinesemittencrab(Eriocheirsinensis):Cloning,tissueexpressionandeffectsofrecombinantpeptideonecdysteroidsecretionofYOs GenCompEndocrinol.2011Sep15;173(3):467-74 | | 6. InhibitionofStriatalSolubleGuanylylCyclase-cGMPSignalingReversesBasalGangliaDysfunctionandAkinesiainExperimentalParkinsonism PLoSOne.2011;6(11):e27187 | | 7. Molecularanalysis,developmentalfunctionandheavymetal-inducedexpressionofABCC5inzebrafish CompBiochemPhysiolBBiochemMolBiol.2011Jan;158(1):46-55 | | 8. FeedbackcontrolthroughcGMP-dependentproteinkinasecontributestodifferentialregulationandcompartmentationofcGMPinratcardiacmyocytes CircRes.2010Nov12;107(10):1232-40 | | 9. TheSolubleGuanylateCyclaseActivatorBAY58-2667ProtectsagainstMorbidityandMortalityinEndotoxicShockbyRecouplingOrganSystems PLoSOne.2013Aug28;8(8):e72155 | | 10. Stimulationofguanylylcyclase-Dbybicarbonate Biochemistry.2009May26;48(20):4417-22 | | 11. Volumeoverloadinducesdifferentialspatiotemporalregulationofmyocardialsolubleguanylylcyclaseineccentrichypertrophyandheartfailure JournalofMolecularandCellularCardiologyVolume60,July2013,Pages72–83 | | 12. DefiningSpecificityDeterminantsofCyclicGMP-MediatedGustatorySensoryTransductioninCaenorhaBDitiselegans genetics.113.152660v1194/4/885 | | 13. Theroleofnitricoxidesignalinginfoodintake;insightsfromtheinnermitochondrialmembranepeptidase2mutantmice RedoxBIOLOGyVolume1,Issue1,2013,Pages498–507 | | 14. CrossregulationbetweencGMP-dependentproteinkinaseandAktinvasodilatationofporcinepulmonaryartery JournalofCardiovascularPharmacology:November2014-Volume64-Issue5-p452-459 | | 15. Geneticablationofsolutecarrierfamily7a3aleadstohepaticsteatosisinzebrafishduringfasting HepatologyVolume60,Issue6,pages1929–1941,December2014 | NewEastBio我们的理念是Our Vision以信誉求发展，以质量树品牌，为科研服务，为生命尽责
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