NewEast/ALK [21280][100 µL]/21280

价格
¥4380.00
货号:21280
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品牌:NewEastBio
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商品描述

CatalogNumber:  21280
GeneSymbol:  ALK,CD246,NBLST3
Description:  Anti-ALKRabbitPolyclonalAntibody
Background:  Anaplasticlymphomakinase(ALK)alsoknownasALKtyrosinekinasereceptororCD246(clusterofdifferentiation246)isaneuronalorphanreceptortyrosinekinase.ALKisessentiallyandtransientlyexpressedinspecificregionsofthecentralandperipheralnervoussystemsandplaysanimportantroleinthegenesisanddifferentiationofthenervoussystem.AbnormalALK,byfusionwithothergenes,gainingadditionalgenecopiesormutations,canbeoncogenic.
Immunogen:  PurifiedALKprotein,humanorigin.
Applications:  ELISA,WB,IHC
RecommendedDilutions:  ELISA:1:1000-1:5000WB:1:100-1:1000IHC:1:50-1:200
Concentration:  0.2mg/ml
Host:  Rabbit
Format:  Liquid
Clonality:  Polyclonal
Isotype:  
Purity:  Purifiedfromserum
Storagebuffer:
Preservative:  no
Constituents:  PBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,50%glycerol
SpeciesReactivity:  RecognizesALKofvertebrates.
StorageConditions:  Storeat-20°C.Avoidfreeze/thawcycles
DownloadDatasheet:  
Westernblot:  
WesternblotanalysisofrecombinantALKprotein.PurifiedHis-taggedALKproteinwasblottedwithanti-ALKrabbitpolyclonalantibody(Cat.#21280).
Immunohistochemistry:  
Immunohistochemicalanalysisofparaffin-embeddedhumanthyroidcancertissuewithanti-ALKpolyclonalantibody(Cat.#21280).Tissuesampleswerefixedwithformaldehydeandblockedwith1%serumfor15minat37°C.Antigenretrievalwasbyheatmediationincitratebuffer(pH6).Sampleswerethenincubatedwithprimaryantibody(1:50dilution)overnightat4°C.AHRP-conjugatedGoatanti-RabbitIgG(1:50dilution)wasusedassecondaryantibody.
 
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