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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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商品描述
CatalogNumber: 21280 |
GeneSymbol: ALK,CD246,NBLST3 |
Description: Anti-ALKRabbitPolyclonalAntibody |
Background: Anaplasticlymphomakinase(ALK)alsoknownasALKtyrosinekinasereceptororCD246(clusterofdifferentiation246)isaneuronalorphanreceptortyrosinekinase.ALKisessentiallyandtransientlyexpressedinspecificregionsofthecentralandperipheralnervoussystemsandplaysanimportantroleinthegenesisanddifferentiationofthenervoussystem.AbnormalALK,byfusionwithothergenes,gainingadditionalgenecopiesormutations,canbeoncogenic. |
Immunogen: PurifiedALKprotein,humanorigin. |
Applications: ELISA,WB,IHC |
RecommendedDilutions: ELISA:1:1000-1:5000WB:1:100-1:1000IHC:1:50-1:200 |
Concentration: 0.2mg/ml |
Host: Rabbit |
Format: Liquid |
Clonality: Polyclonal |
Isotype: |
Purity: Purifiedfromserum |
Storagebuffer: |
Preservative: no |
Constituents: PBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,50%glycerol |
SpeciesReactivity: RecognizesALKofvertebrates. |
StorageConditions: Storeat-20°C.Avoidfreeze/thawcycles |
DownloadDatasheet: |
Westernblot: |
WesternblotanalysisofrecombinantALKprotein.PurifiedHis-taggedALKproteinwasblottedwithanti-ALKrabbitpolyclonalantibody(Cat.#21280). |
Immunohistochemistry: |
Immunohistochemicalanalysisofparaffin-embeddedhumanthyroidcancertissuewithanti-ALKpolyclonalantibody(Cat.#21280).Tissuesampleswerefixedwithformaldehydeandblockedwith1%serumfor15minat37°C.Antigenretrievalwasbyheatmediationincitratebuffer(pH6).Sampleswerethenincubatedwithprimaryantibody(1:50dilution)overnightat4°C.AHRP-conjugatedGoatanti-RabbitIgG(1:50dilution)wasusedassecondaryantibody. |
RelatedProducts | |||||
Catalog# | Name | Size | Price | DataSheet | |
26151 | Anti-ALK(R1275Q)MouseMonoclonalAntibody | 100μL | $495 | ||
21280 | Anti-ALKRabbitPolyclonalAntibody | 100μL | $219 | ||
26226 | Anti-ALK(C1156Y)MouseMonoclonalAntibody | 100μL | $495 |
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