NewEast Biosciences 主营：专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。 华东地区代理NewEastBio专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。

ProductHighlightConfiguration-specificmonoclonalantibodythatrecognizesG i-GTP,butnotG i-GDPTheonlyavailableG iactivationassaykitdirectlymeasuresthelevelofG i-GTPProvidingreliableresultswithconsistentreproducibilitythanotherG iactivationassaykitsbasedoncertaindownstreamsignalingpathwaysProvidingshorterexperimentaltimeCouldbeusedtomonitortheactivationofG iincellsandintissuesbyimmunohistochemistryProductDescription

Astructurallydiverserepertoireofligands,fromphotonstolargepeptides,activatesGprotein-coupledreceptors(GPCRs)toelicittheirphysiologicalfunctions.Ligand-boundGPCRs,inturn,functionasguaninenucleotideexchangefactorscatalyzingtheexchangeofGDPboundontheG subunitwithGTPinthepresenceofG ,causingthedissociationoftheG subunitfromtheG dimertoformtwofunctionalunits(G andG ).BothG andG subunitssignaltovariouscellularsignalingpathways.Basedonthesequenceandfunctionalhomologies,Gproteinsaregroupedintofourfamilies:Gs,Gi,Gq,andG12.

G ifamilyisthelargestfamilyofGproteins.TheyrelaysignalsfrommanyGPCRstoregualtevariousBIOLOGicalfunctions.TherewerenodirectmethodstomeasuretheactivationofG iproteinsbyreceptors(untilthisassaykit).Mostreportsusedoneofthedownstreampathway,i.e.theinhibitionofadenylylcyclases,asareadout.Alternatively,sensitivitytopertussistoxin(PTX)wasusedasanindicatorofpossIBLeG iproteinsinvovledinasignalingpathway.

NewEastBiosciencesG iActivationAssayKitprovidesadirectmeasurementoftheactivationofG iproteins.ThisisasimpleandfasttooltomonitortheactivationofG i.Eachkitprovidessufficientquantitiestoperform20assays.

NewEastBiosciencesG iActivationAssayKitisbasedonthemonoclonalantibodyspecificallyrecognizingtheactiveGTP-boundG iproteins.ThismonoclonalantibodyhasmuchloweraffinitytowardstheinactiveG iproteins.Therefore,afteractivationbyreceptorsignals,activeGTP-boundG iproteinscouldbeimmunoprecipitatedbythismonoclonalantibodyandfurtherquantifiedbywesternblotwithanotheranti-G iantibody.

AssayPrinciple

NewEastBiosciencesG iActivationAssayKitisanimmunoprecipitation/westernblotassaytomeasurethelevelsofactiveGTP-boundG iproteins,eitherfromcellextractsorfrominvitroGTP SloadedG iproteins.Briefly,theanti-activeG imonoclonalantibodywillspecificallybindtoactiveG iprotein.Thisantibody/G icomplexwillthenbepulleddownbyproteinA/Gagarose.TheprecipitatedactiveG iproteinswillbedetectedbyimmunoblotswithanotheranti-G iantibody.

ProductManualRelatedProductsCatalog#NameSizePriceDataSheetiActivationAssayKit\">80301G iActivationAssayKit20assays$637i-GTP\">26901ActiveG i-GTPMonoclonalAntibody30 L$495iMouseMonoclonalAntibody\">26003Anti-G iMouseMonoclonalAntibody100 L$275iRabbitPolyclonalAntibody\">21006Anti-G iRabbitPolyclonalAntibody100 L$275Publications:1.IdentificationofnovelsignallingrolesandtargetsforGalphaandGbetagammadownstreamoftheinsulin-likegrowthfactor1receptorinvascularsmoothmusclecellsBiochemJ.2013Feb15;450(1):209-192.CRHactivationofdifferentsignalingpathwaysresultsindifferentialcalciumsignalinginhumanpregnantmyometriumbeforeandduringlaborJClinEndocrinolMetab.2012Oct;97(10):E1851-613.CXCR4activationdefinesanewsubgroupofSonichedgehog-drivenmedulloblastomaCancerRes.2012Jan1;72(1):122-324.RegulationofestrADIolandprogesteroneproductionbyCRH-R1and-R2isthroughdivergentsignalingpathwaysinculturedhumanplacentaltrophoblastsEndocrinology.2012Oct;153(10):4918-285.WNT-5AstimulatestheGDP/GTPexchangeatpertussistoxin-sensitiveheterotrimericGproteinsCellSignal.2011Mar;23(3):550-46.HeterotrimericGalpha(i)proteinsareregulatedbylipopolysaccharideandareanti-inflammatoryinendotoxemiaandpolymicrobialsepsisBiochimBiophysActa.2011Mar;1813(3):466-727.Estrogen-andxenoestrogen-inducedERKsignalinginpituitarytumorcellsinvolvesestrogenreceptor- interactionswithGprotein- iandcaveolinISteroidsVolume77,Issue5,April2012,Pages424–4328.Na/HexchangerregulatoryfactorscontrolparathyroidhormonereceptorsignalingbyfacilitatingdifferentialactivationofG(alpha)proteinsubunitsJBiolChem.2010Aug27;285(35):26976-869.Beta-arrestin-butnotGprotein-mediatedsignalingbythe\"decoy\"receptorCXCR7ProcNatlAcadSciUSA.2010Jan12;107(2):628-3210.StructuralbasisforactivationoftrimericGiproteinsbymultiplegrowthfactorreceptorsviaGIV/GirdinMolBiolCell.2014Nov5;25(22):3654-7111.CanonicalandNon-canonicalG-proteinSignalingHelpsCoordinateActinDynamicstoPromoteMacrophagePhagocytosisofZymosanMolCellBiol.2014Nov15;34(22):4186-9912.FattyAcid-bindingProtein5(FABP5)RegulatesCognitiveFunctionBothbyDecreasingAnandamideLevelsandbyActivatingtheNuclearReceptorPeroxisomeProliferator-activatedReceptor / (PPAR / )intheBrainJBiolChem.2014May2;289(18):12748-58

华东地区代理NewEastBio专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。

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