苏州蚂蚁淘生物科技有限公司成立于2017年，是一家专业提供实验室整体采购方案的专业公司，为广大采购和实验室解决了大量问题，几年来已经成为所有合作伙伴最优秀的合作伙伴，我们一直秉承信誉第一的原则，坚持不卖假货，哪怕利润损失也要为客户提供最好的产品

我们已经和国外500多个品牌建立了贸易往来，并在国际上享有相当知名度，现已经成为国外近100多个品牌中国指定代理，并成为其中国最优秀的代理商

MoBiTecGmbH是一家私人控股公司，由StephanDiekmann教授于1987年创立。与Max-Planck学会的密切关系以及德国领先的研究中心之一哥廷根的所在地促进了与某些研究中心的密切合作。德国最具创造力的科学家。此外，MoBiTec一直与欧洲和北美的科学家广泛合作，包括法国巴斯德研究所和荷兰Vrije大学的科学家。通过将许多研究人员的精彩创意商业化，MoBiTec能够提供一系列独特的高质量产品和简化的协议，使实验室生活更轻松，并为实际研究留出更多时间。

MoBiTec的产品包括e。G。用于体内检测蛋白质-蛋白质或DNA-蛋白质相互作用的双杂交系统，用于筛选特定肽配体的噬菌粒展示系统和选择具有特异性结合特性的新蛋白质，方便的LamBDaPS基因组文库用于克隆的多功能DNA载体，表达和分析靶基因，有效表达和纯化重组蛋白的试剂盒，细胞转染试剂和细胞培养工具，固定化和可溶性酶，许多用于基因组学和蛋白质组学研究的产品（例如用于PCR，核酸和蛋白质纯化和分析），众多抗体，细胞因子，生长因子和重组蛋白，优质荧光试剂和试剂盒（如蛋白标记试剂盒，标记抗体，细胞染色试剂，柱和凝胶电泳附件）。

与其自有产品线并行，MoBiTec分销德国多家国际公司的产品，包括荧光探针和研究化学品（AnaSpec，TEFLabs），量子点，各种DNA纯化试剂盒和DNA聚合酶（EdgeBio），诊断工具（ZJBioTech）），凝胶电泳配件（Amresco，ClareChemical），糖生物学产品（DextraLaboratories，Sumitomo），细胞转染试剂（Mirus）以及抗体，蛋白质，底物，精细化学品和分子和细胞生物学试剂盒-特别是病毒学，免疫学，神经生物学，细胞凋亡，信号转导，细胞增殖，细胞毒性和癌症研究（MBLI，AGScientific，Amresco，AdarBiotech，AnaSpec，Echelon）。

MoBiTec提供全面的服务，从清晰完整的产品文档开始，为客户提供个性化的建议和技术服务。MoBiTec产品通过MoBiTec的总部在德国分销，在其他国家由分销商分销。此外，MoBiTec不断寻求新产品和可销售的许可技术，因此对科学合作非常感兴趣，以开发创新的产品理念。

性能参数

产品名称：卓越性能，Mobitec巨大芽孢杆菌表达系统英文名称：BacillusmegateriumExpressionSystems产品型号：kit产品规格：kit产品产地：德国品牌商标：MOBITEC价格：面议元

产品详细描述

说明书(bmeg_handbook.pdf)

Features:

• Stable,high-yieldproteinexpressioninBacillusmegaterium
• Idealforbothsmallandlarge-scaleproteinproduction
• Tightlyregulatedandefficientlyinduciblexyloseoperon(upto350-fold)
• Noalkalineproteasesactivityevenupto5hoursafterinduction
• Noendotoxinsobserved
• Versatilecloning(extendedpolylinker,additionalBsrGIsite*)
• Versatileproduction(intracellularorextracellular)
• Versatilepurification(native,6xHis-tag,Strep-tag,Strep/6xHisdouble-tag)
• Removeabletagversions(forTEVorXaproteases)available
• CompatiblewithallB.subtilisvectors

*BsrGIsiteisnotincludedinallvectors.

TheB.megateriumexpressionsystemprovidesaversatileandeasy-to-handletoolforstableandhigh-yieldproteinproduction,bothsmallandlargescale.

B.megateriumhasproventobeanexcellentalternativehosttoE.coliforheterologousgeneexpression.Unlikeotherbacillistrains,proteolyticdegradationbyalkalineproteasesisavoided.Inaddition,therearenoendotoxinsfoundinthecellwall.

HighProteinYieldAsaresult,proteinyieldsareexceptionallyhighevenifinexpensivesubstratesareused.Forexample,theproteinsmutarotase(Mro)andglucosedehydrogenase(Gdh)havebeenaccumulatedupto20%and30%ofthetotalsolubleprotein,respectively.Usingthetightlyregulatedxyloseoperon,thegeneswereinduced130-to350-foldwithoutproteolysis.

VersatileSystemwithaWideRangeofVectorsMoBiTecprovidesawiderangeofusefulvectorsfortheB.megateriumsystemthatareadaptableformostapplicationsandproteinpurificationmethods.Theseincludevectorscarryingasecretionsignalpeptidesequence,a6xHis-tag,aStrep-tag,andaStrep/6xHisdouble-tag.Additionally,allvectorscontainthetightlyregulatedandhighlyinduciblexyloseoperonpromoter.

B.megateriumprotoplastsspecificallyoptimizedfortransformationTheprotoplastssuppliedbyMoBiTecarefromB.megateriumstrainWH320developedbyProf.Dr.W.HillenattheInstituteofMicrobiologyinErlangen,Germany.Theseprotoplastsarepreparedaccordingtoanoptimizedprotocolresultinginthehighesttransformationefficiencies.ForsecretionvectorsweofferprotoplastsofstrainMS941.BothstrainsaremutantsofDSM319,whereWH320isachemicallymutant,whileMS941hasadefineddeletioninthegeneofthemajorextracellularproteaseNprM.

Examples:

SelectedProteinssuccessfullyover-producedinBacillistrainswithourB.megateriumvectors:

• β-Galactosidase(LacZ)¹
• Catabolitecontrolprotein(CcpA)^2,3
• ClostridiumdifficiletoxinA⁴
• Cobaltochelatase(CbiX)⁵
• Dextransucrase⁶
• Endolevanase(LevB)⁷
• Glucosedehydrogenase(GdhA)¹
• Heatshockprotein(HPr)fromPTS(phosphotransferasesugartransportsystem)⁸
• Humansingle-chainurokinase-likeplasminogenactivator(rscuPA)¹
• Levansucrase⁹
• Mutarotase(Mro)¹
• Neopullulanase¹⁰
• TranslocationATPaseofthepreproteintranslocase(SecA)¹¹
• Trehaloserepressor(TreR)¹²

• 药准字：
• 主要组成成分：mobitec的巨大芽孢杆菌表达系统
• 性状：mobitec的巨大芽孢杆菌表达系统
• 适应症：mobitec的巨大芽孢杆菌表达系统
• 规格：mobitec的巨大芽孢杆菌表达系统
• 贮藏：mobitec的巨大芽孢杆菌表达系统
• 有效期：36
• 生产企业：德国

hp-vector表达系统megaterium芽孢杆菌hp-vectorexpressionsystemsforbacillusmegateriumfeatures:highperformancevectorswithoptimizedsequenceproteinyieldupto10timesenhancedcomparedtoproteinexpressionwithbasicplasmidplasmidswithestablishedmcsencodingc-orn-terminalhis-tagforversatilepurification(native,6xhis-tag)secretionwithlipaoryochsignalpeptideupto9foldincreasedoptimizedhighperformance(hp)vectorsforbacillusmegaterium!mobitecprovidesawiderangeofusefulvectorsfortheb.megateriumsystemthatareadaptabletomostapplicationsandproteinpurificationmethods.theseincludevectorscarryingasecretionsignalpeptidesequenceanda6xhis-tag.withournewhp-vectorsyieldsupto10-foldhighercomparedtothebasicplasmidsarepossible.allplasmidshaveestablishedmultiplecloningsites(mcs)forversatilecloning.furthermoreweoffervectorsencodingc-orn-terminalhis-tagsforeasypurification.theproteinsecretionwithlipaoryochsignalpeptidesisuptonine-foldincreased.inductionofproteinexpressionofallvectorsisachievedbythetightlyregulatedandefficientlyinduciblexyloseoperon.versatilesystemwithawiderangeofvectorstheb.megateriumexpressionsystemprovidesaversatileandeasy-to-handletoolforstableandhigh-yieldproteinproduction,bothsmall-andlarge-scale.b.megateriumhasproventobeanexcellentalternativehosttoe.coliforheterologousgeneexpression.unlikeotherbacillistrains,proteolyticdegradationbyalkalineproteasesisavoided.inaddition,therearenoendotoxinsfoundinthecellwall.b.megateriumprotoplastsspecificallyoptimizedfortransformationtheprotoplastssuppliedbymobitecarefromb.megateriumstrainwh320,developedbyprof.dr.w.hillenattheinstituteofmicrobiologyinerlangen,germany.theseprotoplastsarepreparedaccordingtoanoptimizedprotocolresultinginthehighesttransformationefficiencies.forsecretionvectorsweofferprotoplastsofstrainms941.bothstrainsaremutantsofdsm319,wherewh320isachemicallymutant,whilems941hasadefineddeletioninthegeneofthemajorextracellularproteasenprm.orderinformation,shippingandstoragepleaseclickontherespectiveorder#forfurtherdetails.order#descriptionamountbmeg02bacillusmegateriumprotoplastsreadyfortransformation(strainwh320;1)materialissufficientfor4transformationspluscontrolexperiment.5x500µlbmeg50bacillusmegateriumprotoplasts,strainms9415x500µlbmeg31pc-his1623hpshuttlevector,c-term.6xhis-tag;lyophilizeddna10μgbmeg32pn-his-tev1623hpshuttlevector,n-term.his-tag,tev-site10μgbmeg30p3stop1623hp,shuttlevector,lyophilizeddna10μgbmeg33psplipa-hp,shuttlevectorwithsignalsequence,lypophilizeddna10μgbmeg34pspyoch-hp,shuttlevectorwithsignalsequence,lypophilizeddna10µgshippedatrt,protoplastsshippedondryicestorelyophilizedvectorsat4°c,reconstitutedvectorsat-20°c,protoplastsat-70°callvectorsaree.coli/b.megateriumshuttlevectorspleasenotethatprotoplastsofbacillusmegateriumhavealimitedshelflifeofsomemonthsonly,dependingonthedateofproduction.thereforepurchaseanduseofprotoplastsmustbethoroughlyplanned.

苏州蚂蚁淘生物科技有限公司专注于为科研工作者提供CST，Abcam，Sigma，biorbyt，Abclonal，AbDSerotec，GeneTex，ENZO，ScienCell，BI，Swant，emfret，Biovision，Roche，Millipore，BD，Life，eBioscience，Thermo，Qiagen，Santa，Peprotech，R&D，ImmunoStar，GenStar，GeneCopoeia，Cusabio，Bioss，NeoBioscience

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