Description:

Product Source

An E. coli strain that carries the cloned BsgI gene from Bacillus sphaericus B922

Reagents Supplied

The following reagents are supplied with this product:

	Store at (°C)	Concentration
S-adenosylmethionine (SAM)	-20	32 mM
CutSmart® Buffer	-20	10X

Notes:

BsgI requires 80 µM S-adenosyl-methionine in reaction mixture for optimal activity (supplied with enzyme). Incubation without S-adenosylmethionine results in 25% activity.Storage of SAM: S-adenosylmethionine is stored at –20°C as 32 mM solution dissolved in sulfuric acid (0.005 M) and 10% ethanol. SAM in this solution stored under ideal conditions remains active for up to 6 months. SAM is unstable at (pH 7.5), 37°C, and should be replenished for reactions incubated longer than 4 hours. Many problems in achieving complete digestion can be alleviated by using fresh SAM.SAM should be kept frozen at shipping concentration and diluted prior to each reaction.

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