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商品描述
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RNAzol®RTisthemosteffectivereagentforisolationoftotalRNAandsmallRNAfromsamplesofhuman,animal,plant,bacterialandviralorigin.Thispatentedreagent(1)provideshigheryieldandqualityofisolatedRNAthanpreviousreagentsbasedonthesingle-stepmethod.RNAzol®RTisolatespureandundegradedRNAthatisreadyforRT-PCRwithoutDNasetreatment.(2)
- Nochloroform-inducedphaseseparationisnecessarytoobtainpureRNA.JustaddwatertoremoveDNA,proteins,polysaccharidesandothercontaminants.
- Theisolationprocedurecanbecompletedinlessthanonehourandisperformedatroomtemperature,includingallcentrifugationsteps.
- RNAzol®RTisolatestotalRNA,orlargeRNAandsmallRNAinseparatefractions.ThelargeRNAfractioncontainsrRNAandmRNA.ThesmallRNAfractioncontainstRNA,smallRNAandmicroRNAdownto10bases.
- TheisolatedRNAisreadyforRT-PCR,qRT-PCR,microarrays,polyA+selection,northernblotting,RNaseprotectionassayandothermolecularBIOLOGyapplications.
- Duetotheremovalofimpurities,theRNApelletsaresmallerandsolubilizemoreeasilythanpelletsobtainedfromprevioussingle-stepreagents.
- Inaddition,RNAzol®RTallowsforthesimultaneousisolationofRNAandDNA.
RNAzol®RTisusedtoisolateRNAfromtissues,cells,liquidsamplesorblood.Onemilliliterissufficienttoprocessupto100mgtissueyielding50–700μgoflargeRNA(>200bases)and8–120μgofsmallRNA(200–10bases).
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References
- USpatents7,794,932and8,367,817.Internationalpatents.
- Chomczynski,P.etal.RNAzolRT:Anewsingle-stepmethodforisolationofRNA.NatureMethods.December,2010.
