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商品描述
GlycatedLDL | |
| Manufacturer/Trade: | Exocell/Glycacor |
| CatalogueNumbers: | 1009StripPlate |
| Methodology: | IndirectCompetitiveELISA |
| Summaryofprocedure: | Non-rADIoactiveimmunoassayforthequantitativedeterminationofglycatedLDLinplasmatoevaluateglycemiccontrolindiabetes,andtoassessthecontributionofthemodifiedproteintovasculardisease.TheassaydependsuponES12,amonoclonalantibodydirectedagainstaspecificepitopeinglycatedLDL.TheglycatedLDLismeasuredinapolipoproteinBequivalents,andisexpressedasug/mlormg/dl.GlycacorisacompetitiveELISAinamicroplateformat. Tocompletetheassay,theES12isaddedtoamicrotiterplatethatcontainsimmobilizedglycatedLDLandsolubleglycatedLDL(standardorexperimentalsample).ThemonoclonalantibodybindstoeithertheimmobilizedglycatedLDLorthesolublephaseglycatedLDLduringtheprimaryincubation,hencethenotionofcompetitivebinding.Reactantsinthefluidphasearesubsequentlywashedaway,andcapturedES12onthesolidphaseisdetectedwithanenzymeconjugatedanti-mouseantibody.TheresultsaremadequantitativeafterchromogenicreactionwithTMB. |
| SpecimenRequired: | 20ulCitrateorEDTAtreatedplasma |
| AssayRange: | 3-40ug/ml(correspondingto0.3-4mg/dl) |
| Precision: | IntraandinterassayprecisionofsampleswithintheusefulrangeoftheassayhaveaC.V.<10%. |
| Specificity: | ES12isspecificforglycatedLDL,anddoesnotcross-reactwithotherhumanplasmaproteinsincludingnon-glycatedLDLineitherELISAorWesternBlotformats. |
 ©2012ExocellInc. | |
