品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
GlycatedAlbumin(Glycoalbumin) | |
| Manufacturer/Trade: | Exocell/Glycaben |
| CatalogueNumbers: | Glycaben:1003StripPlate |
| Methodology: | SandwichELISA |
| Summaryofprocedure: | Glycabenisanenzymelinkedimmunosorbentassayforthequantitativedeterminationofglycoalbumininhumanplasma.Itprovidesinformationconcerningglycemiccontrolanalogoustothatofferedbyglycohemoglobinmeasurements,exceptthattheproteinmeasuredisglycatedalbuminwhichhasashorterhalf-life. Theassaydependsuponamonoclonalantibody,A717,thatspecificallyrecognizestheglycatedmoietiesinglycatedalbumin.Inpractice,standardsorsamplesareaddedtoA717coatedplates(Glycaben).Theglycoalbuminintheseanalytesbindstooriscapturedbytheantibodyonthesolidphase,andunboundmaterialsarewashedaway.Asubsequentincubationwithanenzymeconjugatedantihumanalbuminantibodyleadstodetectionoftheboundmolecules.BoundconjugateisdeterminedcolorimetricallyafterreactionwithaTMBsubstratesolution. Byrelatingsampleresponsewiththatforthestandards,theconcentrationofglycoalbuminmaybedetermined.Aseparateanalysisoftotalalbuminallowstheresultstobeconvertedtopercentoftotalalbuminthatisglycated. |
| SpecimenRequired: | 50ulcitrateorEDTAtreatedplasma |
| AssayRange: | 0.1-3mg/mlglycatedalbumin |
| Sensitivity: | 0.1mg/ml |
| Precision: | Intra-andinterassayprecisionofsampleswithintheassayrangehaveaC.V.<10%. |
| Specificity: | ThemonoclonalantibodyA717isspecificforglycatedalbumin,anddoesnotcross-reactwithotherproteinsthatoccurinhumanplasmaincludingnon-glycatedalbumin.Theenzymeconjugatedantibodypreparationisspecificforhumanalbumin. |
| ExpectedValues: | Plasmafromnormalnon-diabeticindividualhave0.4-2.4%glycatedalbuminwhereasthatfromuncontrolleddiabeticswillbe>2.5%. |
 ©2012ExocellInc. | |
