NeuroVue^® Dye Filter is useful for neuronal tract tracing studies of up to 3-4 weeks. Spectrally compatible with eGFP and YFP in many systems, and NeuroVue^® Maroon, & Jade. Spectral unmixing required for use with NeuroVue^® Red.

1cm² filter coated with the lipophilic reddish orange emitting dye, NeuroVue^® Orange

(ex max=550nm; em max=570nm).

Advantages:

• Convenient, ready-to-use coated filter format
• More precise control of dye insertion point
• No messy oils, pastes or hard-to-position crystals
• Diffusion properties comparable to or better than other commercially available neurotracing dyes
• More focal results (e.g. labeling of small sets of axons within a pathway)
• Available in multiple colors, including far red, for multi-tract tracing and improved results even in tissues with high myelin expression

Recent Publications:

Diffusion and Imaging Properties of three new lipophilic tracers, NeuroVue^® Maroon, NeuroVue^® Red and NeuroVue^® Green and their use for double and triple labeling of neuronal profile. B Fritzsch et al. Brain Res Bull., 66, 3, 249-258 (2005).

A disorganized innervation of the inner ear persists in the absence of ErbB2. JK Morris et al. Brain Res.,1091:186-199 (2006).

Deafferentiation induces novel projections in the auditory brainstem after hearing onset. CY Hsieh and KS Cramer. Journal of Comparative Neurology, 497:589-599 (2006)

Al-Mehdi AB, Patel M, Haroon A, Reed D, Ohlsson-Wilhelm B, Muirhead K and Gray BD. Increased Depth of Cellular Imaging in the Intact Lung using Far Red and Near Infrared Fluorescent Probes. Int. J. Biomed Imaging. 1-7 (2006).

Stewart CC, Woodring ML, Podniesinski E and Gray BD. Flow cytometer in the infrared: inexpensive modifications to a commercial instrument. Cytometry, Part A, 67A, #2, 104-111, 2005

NeuroVue is a registered trademark of PTI Research, Inc. For patents see list.

下面将主要介绍polysciences 线性PEI(#23966)的产品特性及其溶液的配制过程。 1. 产品特性: 产品名称：线性聚乙烯亚胺(PEI) CAS号 9002-98-6，26913-06-4 分子式 (CH2CH2NH)n 分子量 25000 熔点 73-75°C 外观 白色或黄色固体 结构式   溶解性 溶于热水，低pH值的冷水，甲醇和乙醇；不溶于：苯，aether和丙酮 储存 室温   2. 溶液配制 此指导说明书是经polysciences公司研究，测试及其客户反馈的后编写的。下面推荐一种简单适用而的方法，讲述如何配制浓度为1mg/mL的转染试剂的配制。 材料： 1g #23966、1L 纯水（Milli-Q®纯水，注射用水（WFI），或类似的生物级水）、12 M盐酸、10 M氢氧化钠、一次性0.1-0.2µm PES真空无菌过滤器、无菌高聚乙烯或聚丙烯试剂瓶。 器材： 1 L 玻璃烧杯、1 L玻璃量筒、核准pH计、2支1 mL塑料移液管、托盘、聚四氟乙烯（PTFE）搅拌棒、真空泵 配制过程： 1g #23966——900mL水溶解——搅拌、加热（60-80°C）——加HCl调pH到2.0左右——盖上烧杯，搅拌3h（直至粉末完全溶解）——冷却至室温——加NaOH调pH到6.9-7.1——移液到量筒，加水补足至1L——真空过滤消毒——分装，-20°C保存。 备注：加热或加酸都有助于PEI溶解。当然酸可能不用加那么多，详细用量可试加一点搅拌看看，足够溶解就行，不一定需要加到pH那么低。 试剂储存： 冷冻部分溶液可在-20°C保存长达一年；部分解冻的溶液可在4°C保存2周，不可反复冻融。 粉末储存： 未开封的粉末有效期两年。可在室温下保存直至使用。