StABIlizestoreandshippurifiedDNAatroomtemperature
- Long-termstabilization,storageandshipmentofDNAatroomtemperature
- HighyieldDNArecoveryandusewithoutfurtherpurification
- CompatIBLewithmostdownstreamapplications,e.g.,PCR,sequencing,andmicroarrays
- Availableinavarietyofcompactformats,e.g.,tubes,multi-wellplates&custom
- Cost-effective,eco-friendlysolutiontofreezercrawlandlabspacemanagement
Product
DNAstāble™enablesyoutopreserve,storeandshipyourpurifiedDNAsamplesatambienttemperatures.Samplerecoveryisachievedwithinminutesbysimplyaddingwaterorbuffertothematrix.Sampleisreadyfordownstreamanalysiswithoutneedforanyadditionalpurificationstep.
| Properties | Biomatrica | Unprotected | ColdChain |
|---|---|---|---|
| Yield | |||
| Stability | |||
| QualityofAmplification | |||
| LowConcentrationDNA | |||
| ShortTermStorage<1> | |||
| LongTermStorage>1year | |||
| ShippingRobustness | |||
| CostEffectiveness |
DNAstabilizationcanbeachievedintwoformats:
Pre-driedintubesorplates(DNAstable)
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Data
SuccessfulPCRafterlongtermstorageinDNAstable:
Figure1.Aliquots(1ng)ofpDNA,eitherstoredinDNAstable(DS)orunprotected(NP)for26monthsatambienttemperature(RT)orat60°Cunderacceleratedagingconditions(equivalentto30yearsofroomtemperaturestorage)wereanalyzedbyPCRamplificationusingpUC19specificprimersgeneratinga490bpamplicon.ReferenceDNAsamples(1ng)werestoredat-20°C(control).
StabilityinLiquid-to-Dry(LD)Format:
Figure2.Twodifferentaliquots(20ng)ofgDNAstoredinDNAstable(DS)atroomtemperaturewithouthumidiycontrolfor3.5yearswereanalyzedbyPCRamplificationusingβ-actinspecificprimersgeneratinga500bpamplicon.ReferenceDNAsample(100ng)wasstoredat-20°C.
RecoveryofgenomicDNAafter26monthsofstorageinDNAstable
Figure3.GenomicDNAsampleswerestoredfor26monthsinanumberofconditions,recoveredandcompared.Fromlefttoright:Control-20°C;StoredinDNAstable(DS)atroomtemperature(RT);Unprotected(NP)atroomtemperature;StoredinDNAstable(DS)fortheequivalentof30yearsatroomtemperature(exposedtoacceleratedagingconditionsat60°C);5)Unprotected(NP)andstoredfortheequivalentof30yearsatroomtemperature(exposedtoacceleratedagingconditionsat60°C).
qPCRofgenomicDNAstoredfor26monthsinDNAstable
Figure4.qPCRtracesofexperimentsdescribedinFigure3abovewithstandarddeviationdisplayed.
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Specifications
- Productformats:
- 1.7mltubesand96well-plates(dry)
- 2mLand10mLbottles(liquid-to-dry)
- Custom
- Applicationvolume:5to150μl
- Applicationamount:0.001to30μg
- Dryingtime:10min(SpeedVac)toovernight(Laminarhood)
- Recoveryvolume:10to100μl
- Recovery:∼99.9%
- DNAlong-termstorage:30yearsacceleratedaging;∼4years″realtime″
- DNAstabilizationtestingtemperature:Temperature>60°C
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