RNAandDNAFromOneSample

• BestyieldandqualityforbothRNA&DNA
• SuperiortranscriptomestABIlity&fidelity
• Shipsampleswithoutcoldchain
• Proveninhigh-throughputworkflows

Product

PreserveRNAandDNAInstantly

TheRNAgardBloodSystemisdesignedforthecollection,preservationandpurificationofRNAandDNAfromwholebloodsamples.TheRNAgardBloodpreservativereagentinthetubesrapidlypermeatesandlysescellularstructuresandmembranestostabilizeandprotectRNAandDNAfromdegradation.Preservationiseffectivefor14daysatroomtemperatureor1monthat4°C.

ProductComparison

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Data

ProtectRNAinstantlyatambienttemperatures

Figure1.GeneexpressionanalysisofbloodRNApreservationafter3and14daysinRNAgardBlood(RGB).Humanbloodfrom2healthydonorswascollectedinRNAgardtubesorcompetitor’sRNApreservationtubes.RNAwasisolatedoncollectiondayandafter3or14daysofroomtemperaturestorage.Top:Representativeanalysis,forDonor2,ofgeneexpressionprofileofmorethan47000transcripts,usingtheIluminaHumanHT12BeadArray,forRNAextractedfrombloodcollectedinRNAgardBloodorcompetitor’sTubesafter3days(A,C)or14days(B,D)ofroomtemperaturestorage,relativetoRNAextractedfromfreshlycollectedblood,usingRNAisolationmethodsrecommendedforeachproduct.Bottom:Tablesummarizingthemicroarrayresults.Displayedarethenumberofgeneswith>2-foldchangeinexpressionlevelsrelativetoexpressionlevelsonthedayofbloodsamplecollection.

RNAyieldfromBioMaxiBloodRNAPurificationKit

Figure2.RNAyieldanalyzedbyUVspectroscopy.RNAamountsareforwholetube(2.5mLblood)isolatingusingBioMaxiBloodRNAPurificationkitafter14daysofstorageatambienttemperature.

14-dayRNAprotectionatambienttemperatures

Figure3.MultipledonorcomparisonofRNAgardBloodTubes(RGB)versusCompetitorP(P)after14daysofambienttemperaturestorage.200ngofRNAisvisualizedona1.2%agarosegel.BioMaxiBloodRNAPurificationkitwasusedforisolatingRNAfromRNAgardBloodTubes.

RNAgardBloodSystem:ReproducIBLeandRepeatableRNAPurification:RNAPurity(A260/A280)for3Operators(A,B,C)

Figure4.RNApurityobtainedusingtheRNAgardBloodSystemishighlyreproducible.Bloodwascollectedfrom8healthydonors.After3,7or15daysofsamplestorage,RNAwaspurifiedfromduplicatesamples.RNApurityofeachindividualsamplewasdeterminedbyUVspectrophotometry(A260/A280).OnDay3,duplicatesamplesforeachdonorwerepurifiedby3differentoperatorsshowingreproducibilityofoursystem.

StabilityofspecificRNAtranscriptsisolatedwithRNAgardBloodSystem

Figure5.RNAgardBloodTubemaintainsunalteredgeneexpressionfor14daysat18-25°C.BloodfromtwohealthydonorswascollectedinRNAgardtubesandpurifiedwithBioMaxiRNAaftertheindicatedstoragetime.GeneexpressionofC-Fos(A)andIL1-β(B),normalizedto18SrRNA,wasdeterminedbyqPCRattheindicatedstoragetime,relativetoday0samples.

RNAStabilityafter7weeksat4°C

Figure6.RNAyieldofwholebloodfrom5differentdonorswastestedusingtheRNAgardBloodSystemfollowingstoragefor7weeksat4°Cversuscontrol(Day=0).DatashowsthatthequalityandyieldofRNAfollowing7weeksofstorageinRNAgardBloodTubesat4°CwereequivalenttothatofRNApurifiedfromwholebloodonthedayofblooddraw.

Impactoffreeze/thawconditionsontheintegrityofRNAgardBloodTubes

Table1.RNAgardBloodTubesremainintactafterfreezerstoragefor7days.BloodwascollectedfromtwohealthydonorsintoRNAgardBloodTubes.Thetubeswerethenkeptatroomtemperatureforatleast2hoursbeforefreezingdown.Fourdifferentfreezingmethodsweretested,andthetubeswerestoredfrozenasindicatedfor7days.Thetubeswerethawedusingthreedifferentmethods.Notubedamages(cracks,splits,etc.)wereobserved.

Stablegeneexpression>47,000transcripts

TestedonIlluminaHumanHT-12v4Array

TestedonIlluminaHumanHT-12v4Array

GeneTranscript:BCR-ABL
FunctionalTest:RT-qPCR

Functionaltestingwithageneexpression-basedcancerdiagnostictest.

RNAyield(70%higherwithRNAgard)

RNAQuality

RNAIntegrity

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